Algenol Biotech LLC

Algenol Biofuels, Inc.

Kerstin Baier

Heike Enke

Dan Kramer

Ulf Dühring

Alexandra Friedrich

Tuo Shi

Kui Wang

Frank Uliczka

Irina Piven

Masami Inaba

Karl Ziegler

Christine Oesterhelt

Ulf Duhring

John Robert Coleman

R Paul Woods

Frauke Germer

Ulf Duehring

Craig R Smith

Marianne Grundel

Wolfgang Lockau

Ulf DÜHRING

A genetically enhanced cyanobacterial host cell,sp. ABICyanoI, is disclosed. The enhancedsp. ABICyanoI produces a compound or compounds of interest.

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This invention provides a method for the prolonged production of ethanol using a metabolically enhanced cyanobacterium.

Metabolically enhanced cyanobacterium with sequentially inducible production genes for the production of a first chemical compound

The invention provides novel compositions of matter for the production of ethanol from carbon dioxide and water. Particularly, the invention provides photoautotrophic organisms having a first and second genetic modification, wherein the first genetic modification improves the ethanol production from organisms having the second genetic modification.

Genetically modified cyanobacteria for the production of ethanol

One embodiment of the invention is directed to a genetically enhanced cyanobacterium for the production of a first chemical compound, comprising at least one first recombinant gene encoding a first biocatalyst for the production of the first chemical compound, wherein the gene is under the transcriptional control of a Coor Zn-inducible promoter. Such a cyanobacterium can provide a tighter control of the production of the first chemical compound.

This invention provides a metabolically enhanced cyanobacterium for the production of a chemical compound of interest, having at least two first production genes encoding first biocatalysts for the production of the first chemical compound. One of the two first production genes is under the transcriptional control of a first promoter for the first production gene, whereas the other of the two first production genes is under the transcriptional control of a second promoter for the first production gene. The first promoter and second promoter are separately inducible under different conditions and the at least two first biocatalysts catalyze the same chemical reaction. Metabolically enhanced cyanobacteria according to the present invention allow prolonged production of first chemical compounds. Further provisions of the present invention include, inter alia, a method for producing the metabolically enhanced cyanobacterium and a method for producing a first chemical compound by culturing the metabolically enhanced cyanobacterium.

A cyanobacterial host cell,sp., that harbors at least one recombinant gene for the production of a chemical compounds is provided, as well as vectors derived from an endogenous plasmid isolated from the cell.

A genetically enhanced cyanobacterial host cell,sp. ABICyano1, is disclosed. The enhancedsp. ABICyano1 produces a compound or compounds of interest.

One embodiment of the invention provides a metabolically enhanced photoautotrophic, ethanol producing host cell comprising:

Metabolically enhanced photoautotrophic ethanol producing host cells, method for producing the host cells, constructs for the transformation of the host cells, and method of producing ethanol using the host cells

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Kleinmachnow, Germany

Kerstin Baier