Handylab, Inc.

Pacific Biosciences of California, Inc.

Kerry Wilson

Jeff Williams

Kalyan Handique

Patrick Duffy

Sundaresh N Brahmasandra

Eugene Tu

Kandaswamy Vijayan

Corey M Dambacher

Joseph Franklin Rokicki

Devon Cayer

Richard Lecoultre

The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides.

Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples

The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides. The technology still more particularly relates to automated devices for carrying out pipetting operations, particularly on samples in parallel, consistent with sample preparation and delivery of PCR-ready nucleotide extracts to a cartridge wherein PCR is run.

Automated pipetting apparatus having a combined liquid pump and pipette head system

A rack for holding samples and various reagents, wherein the rack may be used for loading the samples and reagents prior to using the reagents. The rack accepts complementary reagent holders, each of which contain a set of reagents for carrying out a predetermined processing operation, such as preparing biological samples for amplifying and detecting polynucleotides extracted from the samples.

Diagnostic apparatus to extract nucleic acids including a magnetic assembly and a heater assembly

Rack for sample tubes and reagent holders

A holder for reagents, such as may be used for transporting the reagents and for carrying out processing operations on biological samples with the reagents. The holders typically hold reagents for amplifying polynucleotides extracted from the samples. The holder comprises a connecting member; a process tube affixed to the connecting member and having an aperture located in the connecting member; at least one socket, located in the connecting member, and configured to accept a pipette tip; two or more reagent tubes disposed on the underside of the connecting member, each having an inlet aperture located in the connecting member; and one or more receptacles, located in the connecting member and each being configured to receive a reagent tube. Also described are reagent tubes configured with stellated shaped patterns, on their bottom interior surfaces, configured to facilitate complete or near-complete withdrawal of fluid from the tube, via a pipette tip.

Reagent holder, and kits containing same

Housing

Growing community of inventors

Elkhart, IN, United States of America

Kerry Wilson