Commonwealth Scientific and Industrial Research Organisation

Kerrie-Ann Smyth

John Graham Oakeshott

Robyn Joyce Russell

Peter Malcolm Campbell

Thomas Mark Boyce

Richard David Newcomb

Geoffrey Charles De Quetteville Robin

Jeremy Colin Brownlie

Charles Claudianos

Geoffrey Charles Robin

Anthony Gerard Parker

The present invention provides an isolated DNA molecule encoding Malathion Carboxylesterase capable of hydrolysing at least one organophosphate selected from the group consisting of carboxylester organophosphates and dimethyloxon organophosphates. The DNA molecule comprises a nucleotide sequence having at least 60%, preferably at least 80% and more preferably at least 95% homology with Lc&agr;E,, in which the protein encoded by the DNA molecule differs from E,at least in the substitution of Trip at position,with an amino acid selected from the group consisting of Leu, Ser, Ala, Ile, Val, Thr, Cys, Met and Gly. The preferred substituents are Leu and Ser.

Malathion carboxylesterase

Enzyme based bioremediation

Growing community of inventors

Syracuse, NY, United States of America

Kerrie-Ann Smyth