Meiji Seika Pharma Co., Ltd.

Canon Kabushiki Kaisha

Meiji Seika Kaisha, Ltd.

Mitsui Chemicals Crop & Life Solutions, Inc.

Kentaro Yamamoto

Hiroyuki Anzai

Masaaki Mitomi

Kazuhiko Oyama

Mariko Tsuchida

Nobuto Minowa

Naomi Sumida

Kimihiko Goto

Fumio Hata

Koichiro Murashima

Toshimitsu Tanaka

Sato Aihara

Kouji Yanai

Masahisa Nakada

Ikuya Ohno

Shinzo Kagabu

Hirotaka Nagata

Kenji Umemura

Yukiko Takiguchi

An isolated novel polynucleotide comprising a nucleotide sequence encoding at least one polypeptide involved in biosynthesis of pyripyropene A, a recombinant vector comprising the polynucleotide and a transformant comprising the polynucleotide are disclosed. By the present invention, a pyripyropene A biosynthetic gene useful for production of a novel pyripyropene analog, improvement of productivity of a pyripyropene A-producing bacterium, production of an insecticidal agent for microorganisms, creation of a plant resistant to insect pests or the like are provided.

Pyripyropene a biosynthetic gene

There is provided a method for culturing a microorganism in which a particular polynucleotide or a recombinant vector comprising it/them is introduced with an intermediate compound necessary for biosynthesis of pyripyropene A. The method of the present invention allows for the production of pyripyropene.

Method for producing pyripyropene

There is provided a nucleic acid construct comprising a pyripyropene biosynthetic gene cluster and a marker gene. The nucleic acid construct according to the present invention provides an inexpensive and highly productive method for producing pyripyropene.

Nucleic acid construct comprising pyripyropene biosynthetic gene cluster and marker gene

An objective of the present invention is to provide agricultural and horticultural disease control agents that have potent control effect against plant diseases and, at the same time, have high photostability. The agricultural and horticultural disease control agents comprise a novel substance analogous to MK8383 as an active ingredient.

Substances analogous to MK8383 and agricultural and horticultural disease control agents

In a method for manufacturing a display including a light-transmissive substrate bonded onto a display surface of a display body, a bonding surface of the display body and a bonding surface of the light-transmissive substrate are set in a mutually opposed manner across compression-deformable clearance regulation members. The compression-deformable clearance regulation members are provided between the display body and the light-transmissive substrate. An adhesive, previously applied to one terminal edge of a bonding surface, is spread between the bonding surfaces of both the display body and the light-transmissive substrate. This is done by pressing the light-transmissive substrate against the display body starting from one terminal edge towards an other terminal edge thereof and by gradually decreasing a clearance between the bonding surfaces by serially compression-deforming the clearance regulation member from the one terminal edge towards the other terminal edge.

Display manufacturing method

In a display manufacturing method including bonding of a light-transmissive substrate onto a display surface of a display body, the bonding includes bending the light-transmissive substrate into an arch-like shape with a bonding surface thereof having a convex shape. An adhesive sandwiched is put between a bonding surface of the display body and a top of a bonding surface of the bent light-transmissive substrate. The bend of the light-transmissive substrate is released while moving two rollers from a center of the light-transmissive substrate in a direction of the bend of the light-transmissive substrate towards both terminal edges of the light-transmissive substrate in mutually reverse directions. The two rollers press a non-bonding surface of the light-transmissive substrate against the display body.

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Kawasaki, Japan

Kentaro Yamamoto