Shimadzu Corporation

Kentaro Hizume

Takeshi Fujita

Yoshio Tsunazawa

Ichiro Oda

Atsushi Yajima

Shinji Nagamachi

Yutaka Kuratani

Tadafumi Kamikake

This object aims to disclose a biological multi-directional observation device with measures to avoid an illumination backlight problem taken. In a preferable embodiment of the multi-directional observation device, a two-dimensional detector () is arranged right above a transparent specimen support () and a main image forming lens () is arranged right under the two-dimensional detector (). A fluorescence-side filter () which allows only a fluorescence component from a biological specimen () pass therethrough is arranged, if necessary, between the main image forming lens () and the biological specimen (). Reflection mirrors (M, M) are arranged on the down side of the specimen support (), wherein the reflection mirrors are optional systems for leading a light of an image of the rear side of the biological specimen () to the main image forming lens (). A light source device is provided to irradiate light to biological specimen (). A light source in the light source device is set at a position that is not in direct and indirect viewing fields () of the two-dimensional detector, so that the backlight problem is avoided and a vivid biological multi-directional observation image by the two-dimensional observation image by the two-dimensional detector can be acquired.

Biological image acquisition device

In order to reduce light leak influencing the detection capability of a fluorescence imaging apparatus, an interference filter and an absorption filter on an emission-side filter section are arranged serially in the traveling direction of the fluorescence. The interference filter and the absorption filter that are in use for such an arrangement block the waveband light equivalent to the excitation light irradiated onto a sample while fully transmitting the waveband light equivalent to the fluorescence.

Fluorescence imaging apparatus and method for detecting fluorescent image

Disclosed herein is a fluorescence measurement device for a living body configured to be able to reduce wavelength components that will become leak light and to easily switch a wavelength and an irradiation direction. The fluorescence measurement device for a living body comprises: a sample holder on which a living body sample is to be placed; an exciting light-irradiating device having a plurality of exciting light sources arranged at mutually different positions, each of which is composed of a laser diode or a light-emitting diode and is provided with a filter having an optical property to eliminate, from a spectrum of the exciting light source, disturbing wavelength components overlapping with wavelength components of fluorescence to be detected; an electrical switch for switching lighting of the exciting light sources; a detector for picking up an image produced by fluorescence emitted from the sample placed on the sample holder; and an image display device for displaying the image picked up by the detector.

Fluorescent measurement device for living body and exciting light-irradiating device for fluorescent measurement

Growing community of inventors

Kyoto, Japan

Kentaro Hizume