Hamamatsu-photonics K.k.

Hamamatsu University School of Medicine

Hamamastu Photonics K.k.

Kentaro Goto

Toyohiko Yamauchi

Hidenao Yamada

Satoshi Yamamoto

Norikazu Sugiyama

Hidenao Iwai

Takuji Kataoka

Hisayuki Matsui

Satoshi Hirakawa

An interference image acquisition apparatus includes a light source, a beam splitter, a second reflection mirror, an imager, and a first reflection mirror. A cell is placed on one side of a transparent material, and the first reflection mirror is placed on the other side of the transparent material. In a two-beam interferometer, an optical path difference between an optical path length of a first light beam reflected by the first reflection mirror and an optical path length of a second light beam reflected by the second reflection mirror is set to a coherence length of light output from the light source or less. The imager acquires an interference image in a state in which the cell is placed at a position conjugate to an imaging plane in a first optical system between the imaging plane and the first reflection mirror.

Interference image acquiring device and method for acquiring interference image

An evaluation method is a method for evaluating a cell mass containing a plurality of aggregated cells, and includes an imaging step of capturing an image of light obtained from the cell mass by irradiating the cell mass with light, an analysis step of setting a reference point for the cell mass included in image data obtained by the imaging step, setting sampling circles centered on the reference point, and determining a parameter for a state of the cell mass based on image data included in regions on the sampling circles, and an evaluation step of evaluating the cell mass based on the parameter.

Cell mass evaluation method and device for analyzing state of cell mass

An aggregated cell evaluation apparatus includes a laser light source, a speckle image acquisition unit, an SC calculation unit, an evaluation unit, and a memory unit. The speckle image acquisition unit acquires a two-dimensional speckle image by forward scattered light generated in aggregated cells by irradiation of the aggregated cells with laser light output from the laser light source. The SC calculation unit calculates a speckle contrast value Kof a speckle image Iat each time t, determines a maximum value Kamong the speckle contrast values Kto K, and normalizes the speckle contrast value Kat each time tby the maximum value Kto obtain a normalized speckle contrast value K'. The evaluation unit evaluates motion of the aggregated cells, based on the normalized speckle contrast value K&#x2032; at each time t.

Aggregated cell evaluation method and aggregated cell evaluation device

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Hamamatsu, Japan

Kentaro Goto