Akoya Biosciences, Inc.

Other

Caliper Life Sciences, Inc.

Kent S Johnson

Peter J Miller

Carla Coltharp

Howard H Barney

The disclosure features methods that include exposing a biological sample to illumination light and measuring light emission from the sample to obtain N sample images, where each sample image corresponds to a different combination of a wavelength band of the illumination light and one or more wavelength bands of the light emission, where the one or more wavelength bands of the light emission define a wavelength range, and where N>1, and exposing the sample to illumination light in a background excitation band and measuring light emission from the sample in a background spectral band to obtain a background image of the sample, where the background spectral band corresponds to a wavelength within the wavelength range.

Multispectral sample imaging

The disclosure features methods that include exposing a biological sample to illumination light and measuring light emission from the sample to obtain N sample images, where each sample image corresponds to a different combination of a wavelength band of the illumination light and one or more wavelength bands of the light emission, where the one or more wavelength bands of the light emission define a wavelength range, and where N&#x3e;1, and exposing the sample to illumination light in a background excitation band and measuring light emission from the sample in a background spectral band to obtain a background image of the sample, where the background spectral band corresponds to a wavelength within the wavelength range.

The disclosure features methods that include obtaining multispectral image information for a sample that includes a fluorescent dye, calculating from an image cube of the information a first spectrum and a second spectrum, and calculating a pure spectrum of the fluorescent dye in the sample based on the first and second spectra, where a relative contribution of light emission from the fluorescent dye to the second spectrum is larger than a relative contribution of light emission from the fluorescent dye to the first spectrum, where calculating the first and second spectra includes identifying corresponding first and second sets of pixel intensity values in the image cube, and where identifying the first set of pixel intensity values includes designating one or more layers of the image cube as a first layer set.

Pure spectrum extraction from biological samples in fluorescence multispectral imaging

A color slide transparency scanner projects an intense stripe of light through a color slide. Red, green, and blue filters successively filter the stripe of image from the slide. In some applications, a neutral density filter adds still more information from dark areas of the slide image. A carriage carrying the slide moves across the light. A CCD array detects the filtered images and converts them to analog electrical signals. Circuitry converts the analog signals to digital.

Growing community of inventors

Cambridge, MA, United States of America

Kent S Johnson