Amano Enzyme Inc.

National Institute of Advanced Industrial Science and Technology

National Institute of Technology and Evaluation

National Research Institute of Brewing

Kensuke Yuuki

Atsuki Toumoto

Kiyoshi Asai

Masayuki Machida

Keietsu Abe

Shigeharu Mori

Naotake Ogasawara

Motoaki Sano

Katsuya Gomi

Hideki Nagasaki

Taishin Kin

Akira Hosoyama

Osamu Akita

Yuji Nakanishi

Satoru Hisahara

Kinya Washizu

Ryoko Mizuguchi

Kei-ichi Ando

Kei-Ichi Ando

Noriaki Tanaka

It is intended to provide a thermostable AMP deaminase originating in a microorganism. Namely, an AMP deaminase having the following characteristics. (1) Catalyzing the reaction: 5'-adenylic acid+HO&#x2192;5&#x2032;-inosinic acid+NH; (2) being stable at a temperature of 65&#xb0; C. or below (in an acetate buffer (pH 5.6)); (3) having a molecular weight of 48,000&#xb1;2,000 in gel filtration; and (4) having the optimum pH value at around 5.6 (in McIlvaine buffer).

AMP deaminase originating streptomyces and utilization thereof

Lysyl oxidase derived from filamentous fungi and a DNA encoding thereof are provided. Lysyl oxidase including a protein described in of the following (a) or (b): (a) a protein having an amino acid sequence set forth in SEQ ID NO: 2; or (b) a protein having an amino acid sequence obtained by modifying a part of the amino acid sequence set forth in SEQ ID NO: 2, and functioning as lysyl oxidase.

Fungus-origin lysyl oxidases

It is intended to provide a strain capable of producing transglutaminase at a high efficiency; and a process for producing transglutaminase by using this strain. A structural gene fromand a promoter and a terminator acting on this structural gene are externally transferred intoto give a transformant. Transglutaminase is produced by culturing this transformant.

Transglutaminase-producing strain

Growing community of inventors

Kakamigahara, Japan

Kensuke Yuuki