Agency of Industrial Science and Technology

Ministry of International Trade & Industry

Kensuke Furukawa

Hidekatsu Maeda

Hideaki Shinshi

Kazunari Taira

Yoshinori Koyama

Noboru Tomizuka

Masanao Oda

A DNA fragment is disclosed which has connected to the downstream side of a first DNA sequence containing a region coding for the first ribozyme RNA a second DNA sequence containing a region coding for second ribozyme RNA capable of cleaving by self-processing the 3'-terminus site of the first ribozyme RNA subjected to transcription. A ribozyme having the 3'-terminus site thereof self-processed is obtained by effecting transcription of a RNA using as a template a recombinant vector obtained by recombination by the use of the DNA fragment.

Hammerhead ribozymes with enhanced stability provided by an additional 3' h

A DNA segment, specifically a thermostable tryptophan synthetase gene originating in the strain of extremely thermophilic Thermus aquaticus T2, characterized by the restriction enzyme map of FIG. 1, and not cleaved by specific restriction enzymes. An extremely thermophilic plasmid vector pYK 105, having the DNA segment and an Escherichia coli plasmid vector pUC 13 incorporated in a cryptic plasmid pTT8.

Thermostable tryptophan synthetase gene and extremely thermophilic

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Tsukuba, Japan

Kensuke Furukawa