Toyota Jidosha Kabushiki Kaisha

Kabushiki Kaisha Toyota Chuo Kenkyusho

Riken Corporation

Jx Nippon Oil Energy Corporation

Yamaguchi University

Toyota Jidosha Kabuhsiki Kaisha

Kabuhsiki Kaisha Toyota Chuo Jenjyusho

Kenro Tokuhiro

Satoshi Katahira

Nobuhiro Ishida

Haruo Takahashi

Chie Imamura

Noriko Yasutani

Nobuhiko Muramoto

Toru Onishi

Nobuki Tada

Rinji Akada

Masana Hirai

Hisashi Hoshida

Satoshi Saitoh

Risa Nagura

Eiji Nagamori

Tohru Ohnishi

Noriko Shisa

Kozue Mutaguchi

Takeshi Uemura

Takao Imaeda

Moriya Ohkuma

Shigeharu Moriya

Osamu Saotome

Satoshi Saito

Yasuo Matsuo

Chikara Miyazaki

A eukaryotic cell having xylose utilization ability. Provided is a protein that has xylose isomerase activity and has an amino acid sequence including, when aligned with an amino acid sequence expressed by SEQ ID NO:1, the 1st to 6th motifs expressed respectively by SEQ ID NOs:2 to 7 from the N-terminus side in the order described, and having, in place of asparagine (N) in an amino acid sequence of the 6th motif, another amino acid.

Protein having xylose isomerase activity and use of same

The promoter of the present invention causes a desired gene to be highly expressed, especially in thermotolerant yeast. The promoter is located upstream of the PIR1 gene or the CTR1 gene on thechromosome and comprises a region controlling expression of the PIR1 gene or the CTR1 gene.

Promoter and use thereof

A yeast of the genusis modified to improve the ethanol yield from xylose by attenuation of at least one gene selected from the group consisting of the ADH1 gene derived from, a gene functionally equivalent to the ADH1 gene, the ADH4 gene derived from, and a gene functionally equivalent to the ADH4 gene.

Yeast mutant of kluyveromyces and method for ethanol production using the same

It is an object of the disclosure of the present description to provide an eukaryotic cell having xylose utilization ability. The disclosure of the present description provides a novel eukaryotic cell having xylose utilization ability by transforming a yeast or other eukaryotic cell using DNA that codes a xylose isomerase from a termite protist.

Xylose isomerase and use thereof

The present invention provides a transformant into which has been incorporated DNA for coding a foreign protein having lactate dehydrogenase activity and provided with pyruvic acid substrate affinity that equals or exceeds the pyruvic acid substrate affinity of the pyruvate decarboxylase inherent in the host organism. Said transformant can stably mass-produce lactic acid inside a host organism having the pyruvate decarboxylase gene.

Method of controlling ethanol production and mass production of lactic acid and transformant therefor

This invention provides a polynucleotide that encodes a protein having lactate dehydrogenase activity and such protein that can be used for producing D-lactic acid. This polynucleotide has the nucleotide sequence as shown in SEQ ID NO: 1 (a), and it hybridizes under stringent conditions with a probe comprising all or part of the nucleotide sequence as shown in SEQ ID NO: 1 or a complementary strand thereof and encodes a protein having D-lactate dehydrogenase activity (b).

DNA encoding a protein having D-lactate dehydrogenase activity and uses thereof

The present teachings relate to an acid-resistant endoglucanase, which is a protein exhibiting excellent endoglucanase activity under acidic conditions. The present teachings provide a protein having the amino acid sequence set forth in SEQ ID NO: 2, a protein having an amino acid sequence with one or more amino acid modifications in the amino acid sequence set forth in SEQ ID NO: 2 and having endoglucanase activity, or a protein having an amino acid sequence with at least 75% homology to the amino acid sequence set forth in SEQ ID NO: 2 and having endoglucanase activity.

Acid-resistance endoglucanase and the use of thereof

The invention provides a promoter that can be used in the presence of an organic acid. The promoter includes DNA with promoter activity of high osmolarity response 7 gene (HOR7 gene), glycelaldehyde 3 phosphate dehydrogenase 2 gene (TDH2 gene), heat shock protein 30 gene (HSP30), hexose transport protein 7 gene (HXT7 gene), thioredoxin peroxidase 1 gene (AHP1 gene), or membrane protein 1 associated gene gene) of yeasts.

Promoter in the presence of organic acid and utilization thereof

Growing community of inventors

Aichi-gun, Japan

Kenro Tokuhiro