Inguran, LLC

Xy, Inc.

Beckman Coulter, Inc.

Iguran LLC

Kenneth Michael Evans

Thomas Boyd Gilligan

Peter Kiesel

Johnathan Charles Sharpe

Clara Gonzalez-Marin

Ramakrishnan Vishwanath

Paul Barclay Purcell

Kris S Buchanan

George C Malachowski

Edward Allan Stanton

Alex N Hegyi

Erik B Van Munster

Juan F Moreno

Todd Karin

Richard W Lenz

James J Salinas

Munster Erik Van

Kris Buchanan

Richard Lenz

Tae Kwang Suh

Jonathan C Sharpe

Daniela Do Amaral Grossi

Todd A Cox

Thomas B Gilligan

The invention provides methods of flow-cytometrically processing sperm cells in which greater than 25% of sperm cells in the population to be processed have abnormal morphology.

Method for improved sperm cell populations

An optical arrangement receives output light emanating from an object disposed within a fluid column that crosses an optical refraction boundary of the fluid column between the object and the optical arrangement. The optical arrangement modifies the output light such that the modified output light has an intensity that is more uniform than the output light.

Light collection from objects within a fluid column

A discrimination system that forms a fluid column and interrogates objects within the fluid column with an excitation source. An optical arrangement collects output electromagnetic radiation emanating from the excited objects disposed within the fluid column and directs the output electromagnetic radiation to a detector. An analyzer reduces the positional dependency of the detected intensity by normalizing the value based on the position of each object.

Systems and method for correction of positionally dependent electromagnetic radiation detected from objects within a fluid column

Methods for processing and sorting sperm are disclosed. Portions of sperm sorting or staining processes may include standardizing sperm samples by adjusting the concentration of the sperm sample to a predetermined concentration and adjusting the pH of the sample to a predetermined value. Sperm may also be stained in a single staining buffer having a DNA selective dye and a quenching dye.

Methods of processing sperm for sex sorting

In flow cytometry, particles () can be distinguished between populations () by combining n-dimensional parameter data, which may be derived from signal data from a particle, to mathematically achieve numerical results representative of an alteration (). An alteration may include a rotational alteration, a scaled alteration, or perhaps even a translational alteration. Alterations may enhance separation of data points which may provide real-time classification () of signal data corresponding to individual particles into one of at least two populations.

Enhancing flow cytometry discrimination with geometric transformation

A method of sorting cells including magnetically coupling a nozzle assembly to a flow cytometer in a predetermined orientation to form a fluid stream having a sheath fluid and a sample fluid. Particles within the sample fluid are oriented and then interrogated at an inspection zone to produces signals representative of emitted or reflected electromagnetic radiation. The electromagnetic radiation is analyzed to classify the cells and the cells are sorted according to their classification.

Nozzle assembly for a flow cytometry system and methods of manufacture

The invention encompasses methods for reducing the proportion of sperm cells with abnormal morphology, and unviable sperm cells, in a sperm cell population.

Cell sorting methods that improve sorting efficiency and productivity by elevating sorting pressures and incorporate certain steps to help the cells better survive such elevated pressures. In the case of sperm, sorting the steps of standardizing sperm samples, staining sperm samples in a single step, calibrating a flow cytometer to place sperm in the leading edge of droplets, and changing a catch fluid distance may be incorporated individually, or in combination to help sperm better survive the sex sorting process.

High pressure sperm sorting and flow cytometer methods

The invention encompasses a rapid and safe preparation method of sperm nuclei, improved sperm nuclei and a method of using the improved sperm nuclei to calibrate a flow cytometer with higher accuracy.

Sperm nuclei and methods of their manufacture and use

This disclosure pertains to analytical instruments and related methods incorporating beam shaping optics for differentiating very bright and closely related signals over a wide range of operating conditions with an improved and uniform performance.

Growing community of inventors

College Station, TX, United States of America

Kenneth Michael Evans