Geneweave Biosciences, Inc.

Roche Molecular Systems, Inc.

Kenneth G Olson

Bruce J Richardson

Ryan Griswold

Diego Ariel Rey

Shaunak Roy

Victor H Yee

Werner Frei

Ryan C Griswold

Soni Shukla

Nikol De Forest

Leonardo Maestri Teixeira

Rick V Stellmacher

Damian S Matthews

Leonardo M Teixeira

Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.

Systems and methods for detection of cells using engineered transduction particles

An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. The housing includes a puncturer that defines a transfer pathway in fluid communication with the reagent volume. A delivery portion of the housing defines a delivery pathway between the transfer pathway and the reaction chamber when the housing is coupled to the reaction chamber. The actuator has a plunger portion disposed within the reagent volume. An engagement portion of the actuator can be manipulated to move the plunger portion within the reagent volume to deform the reagent container. The puncturer can pierce a frangible portion of the reagent container to convey a reagent from the reagent container into the reaction chamber via the transfer pathway and/or the delivery pathway.

Reagent cartridge and methods for detection of cells

Growing community of inventors

San Jose, CA, United States of America

Kenneth G Olson