Gen-probe, Inc.

Biomerieux, Inc.

Kenneth A Browne

Daniel L Kacian

Norman C Nelson

James Russell

Lizhong Dai

Margarita B Kaminsky

Mark E Filipowsky

Michael McClellan Becker

Ali Laayoun

Matthew C Friedenberg

Lionel Menou

Christelle Tora

Aloke R Banerjee

Fred F Hajjar

Aloke Raj Banerjee

Mark E Flipowsky

Nornan C Nelson

Daniel B Kacian

Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.

Methods for quantitative amplification and detection over a wide dynamic range

The present invention relates to reagents for use in deactivating nucleic acids and methods of making and using the same.

Solutions, methods and kits for deactivating nucleic acids

Reaction mixtures for quantitative amplification and detection over a wide dynamic range

Method of making a formulation for deactivating nucleic acids

Methods and compositions for quantitative amplification and detection over a wide dynamic range

Compositions, methods and devices for detecting nucleic acids. The invention particularly regards composite arrays of immobilized amplification primers and hybridization probes. Also disclosed are compositions and methods for covalently immobilizing oligonucleotides and other biological molecules to glass and plastic surfaces.

Device for amplifying and detecting a target nucleic acid

Unitary hybridization probes having stem-and-loop structures, wherein the stem portion of the structure comprises a pair of interactive arms that are substantially prevented from interacting with target polynucleotides. One arm of the invented parallel-stem hybridization probe has a backbone polarity opposite that of the target-complementary loop sequence of the probe. Rather than interacting in an antiparallel fashion, the arms of parallel-stem hybridization probes interact in a parallel fashion. The arms of the invented dual inversion probes interact in a conventional antiparallel fashion, but have backbone polarities opposite that of the target-complementary loop portion of the probe. Arm portions of the inversion probes do not substantially contribute to sequence-dependent stabilization of probe:target hybrids. Incorporating inversion linkages into the structures of these probes dramatically simplifies the process of designing stem-and-loop hybridization probes.

Inversion probes

A process of fragmenting and labeling a synthetic or natural nucleic acid, comprising the steps of providing a mixture containing a nucleic acid, a labeling agent containing a detectable label, and at least one multivalent metal cation in a substantially aqueous solution; chemically fragmenting the nucleic acid in the mixture to produce a multiplicity of nucleic acid fragments; and attaching at least one label to at least one of the nucleic acid fragments to produce a detectably labeled nucleic acid fragment.

Process for labeling a nucleic acid

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Poway, CA, United States of America

Kenneth A Browne