The Green Cross Corporation

Kenmi Miyano

Yoshiro Iga

Hideo Nishimaki

Kenji Tanaka

Kazuo Takechi

Kazumasa Yokoyama

Yahiro Uemura

Takao Ohmura

Motonori Hashimoto

Yutaka Hirao

Shoju Kameyama

Shouju Kameyama

A method of producing a virus-inactivated protein-containing composition from a protein-containing composition which may be contaminated with virus. The method according to the present invention permits production of pharmaceutically safer virus-inactivated protein preparations without spoiling the protein activity.

Production method for protein-containing composition

A stable aqueous thrombin solution containing thrombin and a sugar and an amino acid as a stabilizer is disclosed. According to the present invention, there is provided a stable aqueous thrombin solution.

Stable aqueous thrombin solution

An aqueous solution containing fibrinogen is heated in the presence of at least a sugar, an amino acid and a magnesium salt to thereby inactivate virus(es) possibly contaminating said fibrinogen. According to this process, the inactivation of the contaminating viruses can be achieved while maintaining the activity of the fibrinogen. Thus a highly safe fibrinogen preparation of excellent qualities can be prepared on an industrial scale.

Process for heat treating fibrinogen

An aqueous solution containing thrombin is heated in the presence of at least one stabilizer selected from various sugars to thereby inactivate viruses possibly contaminating said thrombin. According to this process, the inactivation of the contaminating viruses can be achieved while stably maintaining the thrombin. The stabilizing effect of this process can be further enhanced by using an amino acid as a stabilizer in combination with the sugar.

Process for heat treating thrombin

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Osaka, Japan

Kenmi Miyano