Nitto-denko Corporation

Nagoya University

Nitto Electric Industrial Co., Ltd.

Toray Industries, Inc.

Cmic Co., Ltd.

Kenjiro Mori

Tatsuya Konishi

Eri Maeta

Yasuo Kihara

Takashi Kawasaki

Kenjiro Minomi

Shuji Senda

Keiichi Ushiyama

Keisaku Okada

Shohei Horie

Yoshihiro Hayakawa

Masaki Tsukamoto

Takashi Tsuji

Shoichiro Saito

Tetsuo Watanabe

Tsuyoshi Mukobata

Takeshi Saika

Ayako Matsunawa

Takeo Matsumura

Ujiteru Niwa

Takeshi Matsuzaki

Masafumi Iwamoto

Noritaka Suzuki

Kazuya Miwa

Ken Sato

Katsuhiro Kinoshita

Ryuhei Nagao

Takahiko Ito

Keisuke Makino

Toshiyuki Arai

The present invention provides a linker for solid phase synthesis of nucleic acid, which consists of a compound represented by the formula (I) or the formula (II), a support for solid phase synthesis of nucleic acid, which has a structure represented by the formula (III), and a production method of a nucleic acid, which uses the support: wherein each symbol is as defined in the SPECIFICATION.

Linker and support for solid phase synthesis of nucleic acid, and production method of nucleic acid using said support

The invention provides a cut-out method capable of suppressing production of a byproduct, wherein the object RNA oligonucleotide is not cleaved from a universal linker, which is produced in cutting out the object RNA oligonucleotide from the universal support, and capable of increasing the yield of the object RNA oligonucleotide. The RNA oligonucleotide cut-out method includes a step of bringing a universal support supporting an RNA oligonucleotide in contact with an aqueous solution containing alkylamine and a monovalent inorganic salt.

Method of cutting out RNA oligonucleotide

The present invention provides a solid-phase support for oligonucleotide synthesis for synthesizing long chain oligonucleotide, RNA oligonucleotide and modified oligonucleotide at high synthetic quantity and high purity with a low loading amount of a linker. Provided is a solid-phase support for oligonucleotide synthesis comprising a porous resin bead having a monovinyl monomer unit, a crosslinkable vinyl monomer unit and a polyethylene glycol unit and a cleavable linker loaded on its surface, the porous resin bead having a group capable of binding to a carboxy group by a dehydration condensation reaction on its surface, the cleavable linker having a carboxy group, wherein the carboxy group of the cleavable linker is bound to the group capable of binding to a carboxy group, by a dehydration condensation reaction, anda loading amount of the cleavable linker is 1 to 80 &#x3bc;mol/g relative to the weight of the porous resin bead.

Solid-phase support for oligonucleotide synthesis and oligonucleotide synthesis method

The invention provides a universal linker capable of synthesizing nucleic acid having a hydroxy group at the 3' terminal, a universal support carrying the linker, and a synthesis method of nucleic acid using the universal support. The linker contains a compound represented by the formula wherein each symbol is as defined in the specification.

Linker and support for solid phase synthesis of nucleic acid

The present invention relates to a porous resin bead containing a first aromatic monovinyl compound-divinyl compound-(meth)acrylonitrile-second aromatic monovinyl compound copolymer, in which the second aromatic monovinyl compound contains a group capable of binding with a carboxyl group by dehydration condensation reaction, and in which the porous resin bead has a swelling volume in acetonitrile of from 3 to 6 ml/g.

Porous resin bead and method for producing nucleic acid using the same

The invention provides a universal linker capable of synthesizing nucleic acid having a phosphate group at the 3' terminal, a universal support carrying the linker, and a synthesis method of nucleic acid using the universal support. The linker for solid phase synthesis of nucleic acid contains a compound represented by at least one of the following formulae wherein each symbol is as defined in the specification.

The present invention relates to a porous resin bead containing a first aromatic monovinyl compound-divinyl compound-(meth)acrylonitrile-second aromatic monovinyl compound copolymer, in which the second aromatic monovinyl compound contains a group capable of binding with a carboxyl group by dehydration condensation reaction, and in which the porous resin bead has a dry volume of from 2 to 3 ml/g.

The present invention provides porous resin beads containing an aromatic monovinyl compound-divinyl compound-(meth)acrylamide derivative copolymer. Preferably, the copolymer further contains as a structural unit a second aromatic monovinyl compound having a functional group capable of binding to a carboxyl group by a dehydrating condensation reaction.

Porous resin beads and method of producing nucleic acid using the same

The present invention relates to a method for producing a porous resin particle containing an aromatic vinyl compound-aromatic divinyl compound copolymer having a hydroxyl group, the method including: dissolving a monomer mixture containing an aromatic vinyl compound, an aromatic divinyl compound and a (meth)acrylic acid ester having one hydroxyl group within the molecule thereof, and a polymerization initiator in an organic solvent to obtain a solution containing the monomer mixture and the polymerization initiator; suspending the solution in water in the presence of a dispersion stabilizer; and performing a suspension copolymerization. The method of the invention is capable of easily producing a porous resin particle containing an aromatic vinyl compound-aromatic divinyl compound copolymer having a hydroxyl group, that is used as a support for solid phase synthesis and enables efficient nucleic acid synthesis.

Production method of porous resin particle having hydroxyl group

The present invention provides a method of producing a nucleic acid at high reaction efficiency and high reproducibility with a decreased variation in yield and purity among different reaction lots. A nucleic acid synthesis reaction is carried out on a first solid phase carrier capable of supporting nucleic acid synthesis contained in a solid phase carrier mixture comprising the first solid phase carrier and a second solid phase carrier that does not support nucleic acid synthesis.

Method of producing a nucleic acid

The present invention relates to a method for producing a porous resin particle having a functional group X, the method including: dissolving a radical polymerizable aromatic monovinyl monomer and a radical polymerizable aromatic divinyl monomer together with a polymerization initiator in an organic solvent to prepare a monomer solution, dispersing the monomer solution in water in the presence of a dispersion stabilizer to obtain a suspension polymerization reaction mixture, and performing a suspension copolymerization while adding, when 0 to 80% of the entire polymerization time of the suspension copolymerization is passed, a mercapto compound represented by the formula (I):HS&#x2014;R&#x2014;X&#x2003;&#x2003;(I)in which R represents an alkylene group having a carbon number of 2 to 12, and the functional group X represents a functional group selected from a hydroxy group and a primary amino group, to the suspension polymerization reaction mixture; and the porous resin particle obtained by the method.

Porous resin particle having hydroxy group or primary amino group and production method thereof

The present invention relates to a support for solid-phase synthesis, which contains a porous copolymer particle, the copolymer particle containing 25 to 60% by weight of an aromatic-vinyl structural unit, 20 to 55% by weight of a vinyl cyamide structural unit, 5 to 18% by weight of an ethylenically-unsaturated-carboxylic-acid structural unit, and 2 to 15% by weight of an aromatic-divinyl structural unit. The support according to the invention fluctuates little in the degree of swelling depending on the kind of the organic solvent in contact therewith.

Support for solid-phase synthesis and process for producing the same

The present invention provides, as a resin bead which can be developed for use completely different from the use of conventional polystyrene resin beads, a porous resin bead made from a styrene-hydroxystyrene-divinylbenzene copolymer, which contain a hydroxyl group in an amount of 10&#x2013;1000 &#x3bc;mol/g, and further, a production method of a porous resin bead made from a styrene-hydroxystyrene-divinylbenzene copolymer, which contains suspension copolymerization of a styrene monomer, an acyloxystyrene monomer and a divinylbenzene monomer using an organic solvent (containing at least hydrocarbon and alcohol) and water, followed by hydrolysis reaction.

Porous resin bead production method

The present invention provides a method of producing porous spherical particles, which includes suspension polymerization of a multifunctional monomer having two or more vinyl groups and a vinyl monomer having one vinyl group in an aqueous medium in the presence of a porogen and a distribution stabilizer, wherein the distribution stabilizer is polyvinyl alcohol having a saponification degree of 75 to 85 mol %. According to the method of the present invention, generation of particle agglomerates and large particles can be suppressed and porous spherical particles having a desired average particle size and a narrow particle size distribution can be conveniently produced.

Production method of porous spherical particles of vinyl polymer

A production method of a porous resin or an intermediate therefor, which comprises a step of producing a styrene-acyloxystyrene-divinylbenzene copolymer comprising suspension copolymerization of a styrene monomer, an acyloxystyrene monomer and a divinylbenzene monomer using an organic solvent and water, and a step of converting the styrene-acyloxystyrene-divinylbenzene copolymer to a styrene-hydroxystyrene-divinylbenzene copolymer, wherein a chain transfer agent is added to the suspension copolymerization system.

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Ibaraki, Japan

Kenjiro Mori