Juridical Foundation the Chemo-sero-therapeutic Research Institute

The Chemo-sero-therapeutic Research Institute

Glaxosmithkline Biologicals Sa

Km Biologics Co., Ltd.

Kenjiro Kawatsu

Yoshinobu Miyatsu

Hiroshi Mizokami

Toshihiro Maeda

Satoshi Koyanagi

Toshio Murakami

Michele Therese Rita Lecocq

Tetsuro Tanabe

Pascal Charles Louis Gerkens

Tatsuya Kasugaya

Pascal Charles Gerkens

Michele Therese Lecocq

A method of producing a virus in cell culture comprising at least the steps of a) providing a population of cells cultured in a cell culture medium, b) infecting the population of cells by i. inoculating the population with the virus, and ii. incubating the inoculated population so as to allow the virus to replicate and propagate, c) collecting the produced virus, thereby providing a viral harvest, and d) purifying the virus, wherein a power density of at least 15 W/m, at least 30 W/m, at least 60 W/m, at least 100 W/m, at least 120 W/mis applied to the cell culture at least during step b).

Method of viral purification

A method for purifying a modified major mite allergen obtained by the genetic recombination technique and a purified modified major mite allergen obtained by said method for purification are provided. A method for purifying a modified major mite allergen obtained by the genetic recombination technique, which comprises the purification steps: (1) Washing and recovering inclusion bodies containing a modified major mite allergen obtained by the genetic recombination technique with MF membrane; (2) Dissolving said inclusion bodies followed by refolding; (3) Concentrating a solution containing the modified major mite allergen with simultaneous removal of low molecular weight components with ultrafiltration membrane; (4) Recovering the modified major mite allergen in non-adsorbed fractions with an anion exchanger; (5) Recovering the modified major mite allergen in adsorbed fractions with a hydrophobic gel; and (6) Recovering the modified major mite allergen in adsorbed fractions with an anion exchanger, and a modified major mite allergen with high purity obtained by said method for purification.

Method for purifying modified major mite allergen

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Kumamoto, Japan

Kenjiro Kawatsu