Hitachi, Ltd.

Oji Paper Co., Ltd.

Tokyo Medical and Dental University

Japan Science and Technology Agency

Kanagawa Academy of Science and Technology

Jsr Corporation

Hitachi-high-technologies Corporation

Agency of Industrial Science and Technology

Japan Science and Technology Corporation

Ministry of International Trade & Industry

Mitsui Engineering and Shipbuilding Company Limited

Lsi Medience Corporation

Tokyo Ohka Kogyo Kabushiki Kaisha

Mitsubishi Chemical Medience Corporation

On-chip Cellomics Consortium Co., Ltd.

Kenji Yasuda

Kazunori Okano

Masaru Kato

Hirokazu Kato

Yoshitada Oshida

Satoshi Takahashi

Toshihiro Minato

Akihiro Hattori

Toshinari Sakurai

Taisaku Seino

Takeshi Sakamoto

Tomoyuki Kaneko

Junkichi Miura

Akira Kobayashi

Hideyuki Terazono

Toshikazu Nagura

Makiko Tokita

Fumimasa Nomura

Toshihiko Nakata

Hideki Kambara

Kenichi Kawabata

Kazuo Takeda

Shin-ichiro Umemura

Kazumichi Imai

Keiichi Nagai

Chihiro Uematsu

Kenko Uchida

Norio Shimizu

Susumu Hasegawa

Yoshinori Takata

Takashi Irie

Daizo Tokinaga

Tomoharu Kajiyama

Teruaki Kobayashi

Norio Yamamura

Tomoaki Sakata

Yoshinori Harada

Sadabumi Ohnuma

Hiroko Matsunaga

Hiroyuki Takei

Masahiro Nakagawa

Harumi Tsuruta

Atsushi Sugiyama

Mitsuru Tamura

Masahito Hayashi

Hiroshi Arakawa

Masahito Ito

Shinichiro Umemura

Tetsuo Yokoyama

Yasuhiro Yoshimura

Yoshishige Endo

Masao Kamahori

Yasuo Wada

Masahiro Kamiya

Masami Aoki

Atsushi Hiraiwa

Takao Terayama

Kazunori Takahashi

Satoshi Yamashita

Takanori Aono

Takanori Ichiki

Shinichi Fukuzono

Kou Hasegawa

Hideo Nakanishi

Shigeyuki Nakada

Noriaki Kimura

Kazuaki Ohshima

Kazuaki Sasaki

Katsuo Koshimura

Yukiko Ikeda

Tsunehisa Shigetani

Mitsuo Ito

Shozo Toda

Tadaaki Tanaka

Yuichi Wakamoto

Kazuhisa Kodama

Tetsuya Yomo

Masahiko Kitajima

Hisao Maruyama

Noboru Shimada

Norie Maeuchihara

Shinichi Fukuzuno

Yoshihisa Mizuno

Masao Maekawa

Akihide Ito

Toshie Uriu

Akira Umemoto

Michinori Tsuchiya

Takefumi Yokokura

Kentaro Ando

Mamoru Fukushima

Yumiko Abe

Shozo Kokubo

Kunihiko Kaneko

Ippei Inoue

In the present invention, a cardiomyocyte cluster is disposed on a transparent substrate, and the quality of the cardiomyocytes is evaluated from the response of the cells to a forced pulsation stimulus applied to the cardiomyocytes. The cardiomyocyte cluster is disposed on the transparent substrate, and is exposed to the flow of a liquid containing an agent in a manner so that the agent acts on the cells, which configure a network. The extent of cardiac toxicity resulting from the agent is evaluated from measuring the fluctuations obtained from a comparison of adjacent cardiomyocytes of the network.

Method and apparatus for testing cardiotoxicity and evaluating cardiomyocytes

Provided is a device for concentrating and separating cells, which has a function for continuously concentrating cells; a function for then continuously arranging the concentrated cells in predetermined regions of a flow path; a function for simultaneously identifying shape and fluorescent emission in one-cell units on the basis of cell concentration and purification images, which serve to continuously separate and purify cells that have different properties in that they are either attracted to or repelled by an induction electrophoresis force of a predetermined frequency; and a function for identifying cells on the basis of this shape and fluorescent emission information and thereby separating and purifying the cells.

Device for concentrating and separating cells

Provided is a cell concentration and purification device, having: a function of continuously concentrating cells; a function of then subsequently disposing the cells continuously in a specific region of a channel; a function of simultaneously recognizing, based on an image, the shape and fluorescence emission of each single cell; and a function of recognizing the cells and then separating and purifying the same based on the data relating to the shape and fluorescence emission thereof.

Cell analyzer

The present invention provides a liquid reflux reaction control device comprising:

Liquid reflux high-speed gene amplification device

Provided is a device and a method for examining myocardial toxicity, which can be realized in vitro in an equivalent manner as those conventionally carried out in vivo. A cell population as a pulsating pacemaker is arranged on a transparent substrate. Myocardial pulsating cells are arranged while being spaced apart appropriately. Fibroblast cells are arranged with/connected to the myocardial pulsating cells to form a cell network. Each of the myocardial pulsating cells and fibroblast cells forming the network is arranged on a transparent electrode provided on the transparent substrate. The cells forming the network are exposed to a flow of a solution containing a drug and QT delay due to the drug is evaluated.

Device for examining myocardial toxicity, chip for examining myocardial toxicity and method for examining myocardial toxicity

A cell separation apparatus comprises a means for moving a cell within a cell separating space by applying an electrical voltage to cells in the cell separating space using gel electrodes, and channels for separating and discharging the cell, which thus does not damage a cell sample and prevents the exhaustion of an electrode due to its electrolysis.

Cell separation apparatus

A liquid sample flow containing living cells is irradiated with measurement laser light and the photo data of at least either scattering light or fluorescence that is generated by each of the living cells in the liquid sample flow due to the irradiation with the measurement laser light is acquired. Based on the photo data thus acquired, it is determined whether each of the cells assignable to the respective photo data is an unnecessary living cell or a target living cell. Based on the determination results, a pulse voltage is then applied exclusively to the living cells having been determined as unnecessary living cells so that the unnecessary living cells are damaged and killed.

Flow cytometer having cell-sorting function and method of separating living cells

A cell sorting device using an inexpensive chip capable of being exchanged for each sample. The chip includes: a first flow path allowing buffer fluid containing cells to flow down; second and third flow paths which put the first flow path therebetween and allow buffer fluid not containing cells to flow down; a fourth flow path which allows the buffer fluid as a single flow path formed by joining the buffer fluids in the other three flow paths; a cell detecting region for detecting cells flowing with the buffer fluid down the fourth flow path; and a cell sorting region for sorting the cells according to a type of the cells detected. The first to fourth flow paths are cascaded, are supplied with the buffer fluid from reservoirs with the same fluid level, and have substantially the same width or cross-section area.

Cell measuring and sorting chip

A DNA inspecting apparatus including driving means for relatively changing positions of the multi-spot lights and a position of the DNA chip so as to detect the fluorescent lights in such a manner that a desired area on the DNA chip is irradiated with the multi-spot lights, and a control system for determining and inspecting DNA information about the to-be-inspected DNA chip from fluorescent light intensities and fluorescent light positions of the desired area on the DNA chip, the fluorescent light intensities and the fluorescent light positions being detected by the driving means and the fluorescent light detecting means.

Method of inspecting a DNA chip and apparatus thereof

This invention relates to a method and apparatus for processing cell cultures. The apparatus comprises a micro-chamber comprising no more than one absorption layer and at least one gel layer, in this order, laminated on a transparent base plate having no conspicuous absorbency in visible and infrared regions, and at least one light source, the absorption layer having absorbency in visible and infrared regions, and the gel-like material being a substance which has a gel dissolution temperature of 100 degree C. or less, solates when heated and is in a gel state at room temperature and has absorbency for a specific wave length of visible and infrared regions, and the light source having a monochromatic light in the specific wave length, wherein the light source is disposed such that it irradiates on the absorption layer and/or the gel layer, with the exception that when no absorption layer is provided, at least two layers each composed of a gel-like material are laminated on the transparent base plate.

Micro-chamber processing apparatus for cell culture and method

The present invention provides an apparatus for evaluating a drug effect enabling on-chip evaluation of the effect of a drug while the drug is acting on hERG-expressing cells. The present invention also provides a myocardial toxicity test apparatus and method therefor enabling in vitro myocardial toxicity testing that has previously been performed in vivo. A pulsating cell population and hERG-expressing cells (target model cells) are suitably isolated and arranged on a transparent substrate so that the two form gap junctions. The hERG-expressing cells are arranged on transparent electrodes provided on the transparent substrate. The hERG-expressing cells are exposed to a flow of a liquid containing a drug such that the drug acts thereon. The difference between the normal pulsation of hERG-expressing cells and the pulsation when a drug is acting thereon is captured via electric signals obtained from electrodes, and the properties of the change in potential are evaluated.

Model cell chip, apparatus for evaluating drug effect using the model cell chip and method of evaluating drug effect

This invention relates to a method and apparatus for process cell cultures. The apparatus comprises a micro-chamber comprising no more than one absorption layer and at least one gel layer, in this order, laminated on a transparent base plate having no conspicuous absorbency in visible and infrared regions, and at least one light source. The absorption layer has absorbency in visible and infrared regions, and the gel-like material is a substance which has a gel dissolution temperature of 100 degree C. or less, solates when heated and is in a gel state at room temperature and has absorbency for a specific wave length of visible and infrared regions. The light source is monochromatic light in the specific wave length. The light source is disposed such that it irradiates on the absorption layer and/or gel layer, with the exception that when no absorption layer is provided, at least two layers each composed of a gel-like material are laminated on the transparent base plate.

Apparatus and method for machining microchamber for cell culture

A microchamber including a glass substrate which is transparent to a specific wavelength, an absorbent region which absorbs the specific wavelength, and a melting substance region which does not absorb the specific wavelength, is solid at room temperature and melts when heated, which regions are layered on the glass substrate. The absorbent region, is irradiated with a focused light beam of the specific wavelength and locally heated in the vicinity of the converging rays, so that the melting substance region is locally melted at a portion adjacent to the absorbent region, thereby forming a cavity as the focused light beam moves. Accordingly, the shape of the microchamber can be arbitrarily changed in accordance with the process of cell culture.

Cell-cultivation microchamber

In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.

Cellomics system

A fluorescent or luminescence image of at least part of a biological sample is imaged, then a transmission light image of at least part of the biological sample is imaged, and thereafter the fluorescent or luminescence image and the transmission light image are overlaid such that the identical area of the sample is coincided to display. The present invention allows detecting the accurate location of fluorescence or luminescence in a given part of the biological sample.

Biological sample optical measuring method and biological sample optical measuring apparatus

Growing community of inventors

Tokyo, Japan

Kenji Yasuda