Bioleaders Corporation

Unitika Ltd.

M.d. Lab Co., Ltd., Department of Veterinary Science Chung Nam University

Kenji Soda

Makoto Ashiuchi

SeungGoo Lee

JaeJun Song

SeungPyo Hong

YoonHo Choi

SunYoung Shin

MoonHee Sung

JaeSuk Han

DaeHeoun Baek

Moon-Hee Sung

Seung-Pyo Hong

Chul-Joong Kim

Chang-Min Jung

Jong-Su Lee

Toshihisa Ohshima

var., accession no. KCTC 0697BP, a salt-resistantvariant isolated from ',' a traditional Korean fermented soy bean paste. Thisvariant produces poly-gamma-glutamic acid (or poly-gamma-glutamate, &#x3b3;-PGA), an edible, soluble, anionic, and biodegradable high molecular-weight polymer.var.produces &#x3b3;-PGA with a higher molecular weight than other extracellular &#x3b3;-PGAs derived from generalsp. strains. Accordingly, the &#x3b3;-PGA produced byvar.can be utilized for the development of high value-added products, such as cosmetics, absorption agents, and biodegradable plastic materials.

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The present invention relates to a surface expression vector having pgsBCA, a gene coding poly-gamma-glutamate synthetase and a method for expression of target protein at the surface of microorganism using the vector. The vector, in which foreign genes are inserted, transforms microorganisms and makes foreign proteins expressed stably on the surface of microorganisms.

Surface expression vectors having pgsBCA the gene coding poly-gamma-glutamate synthetase, and a method for expression of target protein at the surface of microorganism using the vector

Salt-resistantvar. chungkookjang, accession no. KCTC 0697BP, separated from 'chungkookjang' a traditional Korean fermented soy bean paste and its poly-gamma-glutamic acid (or poly-gamma-glutamate, g-PGA), an edible, soluble, anionic, and biodegradable high molecular-weight polymer.var. chungkookjang produces g-PGA with a higher molecular weight than other extracellular g-PGAs derived from generalsp. strains and can be utilized for the development of high value-added products, such as cosmetics, absorption agents, and biodegradable plastic materials.

Leucine dehydrogenase is disclosed which retains 80% or more of the activity even after it is incubated in a buffer solution at about 70.degree. C. for 10 minutes, as compared to the activity prior to incubation. A process for producing a heat resistant leucine dehydrogenase is also disclosed. The process comprises culturing a microorganism having an optimum growth temperature of 50.degree. to 85.degree. C. and harvesting the leucine dehydrogenase from the culture and a composition of assaying for leucine aminopeptidase (LAP) comprising the heat resistant leucine dehydrogenase. The leucine dehydrogenase can be stored for a long period of time and thus very effective for investigations in biochemistry, food analysis and clinical test. Further the composition provides extremely easy assay for the LAP activity in high accuracy with good reproducibility which is an important item in clinical test.

Leucine dehydrogenase and a process for production thereof

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Kyoto, Japan

Kenji Soda