Ajinomoto Co., Ltd.

Shionogi Company Limited

Yamasa Shoyu Kabushiki Kaisha

Mitsui Toatsu Chemicals, Incorporated

Kyowa Hakko Kogyo Co., Ltd.

Kyowa Hakko Bio Co., Ltd.

Kenji Soda

Hidehiko Tanaka

Nobuyoshi Esaki

Shin-ichi Hashimoto

Hajime Ikeda

Yoshiyuki Yonetani

Makoto Yagasaki

Kenji Inagaki

Akira Kuninaka

Hitoshi Kusakabe

Hiroyuki Inoue

Manabu Sugimoto

Shigeru Nakamori

Yoshio Kawahara

Hidetsugu Nakazawa

Koji Mitsugi

Yuichiro Midorikawa

Kazuhiko Totsuka

Kenjiro Kodama

Masao Shimada

Manabu Yokomori

Yoshiyuki Asai

Haruo Misono

Takeshi Niwa

The present invention provides a protein having low-substrate-specific amino acid racemase activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a process for producing the protein by using the transformant; and a process for producing a racemic amino acid which comprises allowing a culture of the transformant or a treated matter thereof as an enzyme source and an amino acid to be present in an aqueous medium to racemize the amino acid in the aqueous medium, and recovering the racemic amino acid from the aqueous medium.

Low-substrate-specific amino acid racemase and process for producing racemic amino acid

Amino acid racemase having low substrate specificity and process for producing racemic amino acid

A recombinant protein capable of constituting a recombinant L-methionine .gamma.-lyase or a variant thereof, a recombinant oligomeric enzyme, preferably tetrameric enzyme, consisting of the recombinant protein, a DNA molecule encoding the same, an expression vector containing the DNA molecule, a host cell harboring the expression vector, a method of preparing the recombinant L-methionine .gamma.-lyase, and anti-tumor agent comprising the recombinant L-methionine .gamma.-lyase or a variant thereof are provided.

Recombinant L-methionine Y-lyase

Recombinant L-methionine .gamma.-lyase

A process for producing L-lysine, which comprises culturing a mutant L-lysine-producing strain belonging to the genus Brevibacterium or the genus Corynebacterium and having selenalysine resistance in a nutrient medium, producing and accumulating L-lysine in the culture broth and collecting L-lysine from the culture broth.

Process for producing L-lysine by fermentation with a bacteria having

A process for the preparation of L-tryptophan comprises reacting indole with serine in the presence of tryptophan synthetase or tryptophanase, wherein DL-serine or D-serine is used and a serine racemizing enzyme is included in the reaction system and reacted with the serine.

Process for preparation of L-tryptophan by enzyme

An L-lysine .alpha.-oxidase, that is, a novel L-amino acid oxidase having very high substrate-specificity to L-lysine is produced by culturing a specific microorganism belonging to Trichoderma in a medium.

L-Lysine .alpha.-oxidase

L-lysine contained in a sample can be efficiently determined by using the L-lysine .alpha.-oxidase. The preferred enzyme is an L-lysine .alpha.-oxidase, that is, a novel L-amino acid oxidase having very high substrate-specificity to L-lysine is produced by culturing a specific microorganism belonging to Trichoderma in a medium.

Assay of L-lysine

Method for the production of substituted sulfur containing L-amino acids by contacting .beta.- or .gamma.-substituted amino acids in an aqueous medium containing methioninase together with the precursor of the substituent.

Method of producing sulfur containing L-amino acids

Growing community of inventors

Uji, Japan

Kenji Soda