Kyoto University

Astellas Pharma Gmbh

Kenji Osafune

Yasushi Kondo

Kazuwa Nakao

Yukiko Yamagishi

Taro Toyoda

Tatsuya Kawamoto

Nobuya Inagaki

Michiaki Mishima

Shimpei Gotoh

Shinichi Sueta

Tomoko Kasahara

Masatoshi Kajiwara

Azuma Kimura

Masakatsu Sone

Michinori Funato

Isao Ito

Norikazu Nishino

Takafumi Toyohara

Toshikazu Araoka

Hirofumi Hitomi

Maki Kotaka

Shin-ichi Mae

Fumihiko Shiota

Daisuke Taura

Shin-Ichi Mae

A method of producing renal progenitor cells from pluripotent stem cells involves culturing pluripotent stem cells in a medium containing FGF2, BMP4, a GSK-3&#x3b2; inhibitor, and retinoic acid or a derivative thereof, culturing the resulting cells in a medium containing FGF2, a GSK-3&#x3b2; inhibitor, and BMP7, culturing the resulting cells in a medium containing FGF2, a GSK-3&#x3b2; inhibitor, BMP7, and a TGF&#x3b2; inhibitor, culturing the resulting cells in a medium containing FGF2, a GSK-3&#x3b2; inhibitor, BMP7, activin, and a ROCK (Rho-kinase) inhibitor, culturing the resulting cells in a medium containing retinoic acid or a derivative thereof, and FGF9, and culturing the resulting cells in a medium containing a GSK-3&#x3b2; inhibitor and FGF9, to induce renal progenitor cells from intermediate mesodermal cells.

Method for inducing differentiation of intermediate mesodermal cell to renal progenitor cell, and method for inducing differentiation of pluripotent stem cell to renal progenitor cell

A method for acquiring and producing high-purity renal progenitor cells from a renal progenitor cell population into which pluripotent stem cells are induced to differentiate, by identifying a cell surface antigen marker specific to renal progenitor cells. The high-purity renal progenitor cells can be used in regenerative medicine for renal diseases, such as renal failure.

Method for producing renal progenitor cells

The present invention provides: a method for producing renal progenitor cells from intermediate mesoderm cells, which comprises a step of culturing intermediate mesoderm cells in a medium containing a TGF&#x3b2; signaling activator(s) and a BMP inhibitor(s); the renal progenitor cells produced by the method; a pharmaceutical composition comprising the renal progenitor cells; and a therapeutic drug for kidney diseases comprising the renal progenitor cells.

This invention provides a method for stably producing alveolar epithelial progenitor cells from pluripotent stem cells, including steps of culturing pluripotent stem cells in (1) a medium containing activin A and a GSK3&#x3b2; inhibitor, (2) a medium containing a BMP inhibitor and a TGF&#x3b2; inhibitor, and (3) a medium containing BMP4, retinoic acid, and a GSK3&#x3b2; inhibitor.

Method for inducing alveolar epithelial progenitor cells

A method for acquiring and producing high-purity renal progenitor cells from a renal progenitor cell population into which pluripotent stem cells are induced to differentiate, by identifying a cell surface antigen marker specific to renal progenitor cells. The disclosed method may include, for example, the steps of:

A method for producing hepatocytes from hepatoblasts is provided. The method includes the step of culturing the hepatoblasts in a medium containing a compound selected from the group consisting of pregnenolone and an adrenergic agonist. The hepatoblasts can be obtained by culturing endodermal cells in a medium containing DMSO, and the endodermal cells can be obtained by culturing pluripotent stem cells in a medium containing Activin A and a GSK-3&#x3b2; inhibitor. Accordingly, a method for producing hepatocytes from pluripotent stem cells is also provided by employing the method of the present invention.

Method for inducing hepatocytes

Provided is a method for generating pancreatic bud cells, having the step of culturing PDX1/NKX6.1cells in a medium containing KGF, EGF and a BMP inhibitor. The culturing step may be performed in suspension cultures or in adherent cultures. When the cells are cultured in adherent cultures, the cells may be cultured in a medium further containing a ROCK inhibitor or a nonmuscle myosin II inhibitor.

Method for generating pancreatic bud cells and therapeutic agent for pancreatic disease containing pancreatic bud cells

An object of the present invention is to provide a method for inducing the differentiation of pancreatic precursor cells into exocrine pancreatic cells. The above object is achieved by providing a method for inducing the differentiation of pancreatic precursor cells into exocrine pancreatic cells using a culture solution containing a histone deacetylase inhibitor and/or a ligand protein for a Notch signal, and a protein kinase C activator.

Method for inducing exocrine pancreatic cells

The present invention provides a method for inducing differentiation of a human pluripotent stem cell into an intermediate mesoderm cell without the use of a growth factor. Such method comprises performing culture in a medium containing a specific compound, so as to induce differentiation of a human pluripotent stem cell into an intermediate mesoderm cell.

Method for inducing differentiation of human pluripotent stem cells into intermediate mesoderm cells

Provided is a method for inducing pancreatic hormone-producing cells from pancreatic progenitor cells efficiently. The method comprises a step of culturing the cells in a culture medium comprising sodium cromoglicate.

Method for generating pancreatic hormone-producing cells

An object of the present invention is to provide a method for inducing the differentiation of erythropoietin-producing cells from human pluripotent stem cells. The above object is achieved by providing a method for inducing the differentiation of erythropoietin-producing cells from human pluripotent stem cells using a medium containing a specific growth factor and a specific compound.

Method for inducing erythropoietin-producing cell

The present invention relates to: a method for producing an intermediate mesoderm cell from a human pluripotent stem cell, comprising a step of culturing the human pluripotent stem cell in a medium containing Activin A and Wnt or a functional equivalent of Wnt and a step of culturing cells in a medium containing BMP and Wnt or a functional equivalent of Wnt; to a method for producing a metanephric cell from the intermediate mesoderm cell produced by the first method; to a human pluripotent stem cell having a foreign reporter gene in the chromosome wherein the gene is expressed interlocked with the expression of endogenous OSR1; to a method for screening for an inducer for differentiation into intermediate mesoderm using the human pluripotent stem cell; and to a kit for inducing the differentiation into an intermediate mesoderm cell.

Growing community of inventors

Kyoto, Japan

Kenji Osafune