Sumitomo Chemical Company, Limited

Other

Kenji Oeda

Fumiharu Ishige

Yasuhiko Takahashi

Toru Yamada

Eijiro Watanabe

Yasuo Matsumoto

Toshiya Yamamoto

Yuki Nakajima

Satomi Nishikawa

Nam-Hai Chua

Hideo Ohkawa

Masatoshi Shimizu

Keiko Nakamura

Kazuyuki Oshie

Satomi Torikai

The present invention relates to a method for improving symptom of mood disorder or its related disorder comprising a step of allowing Gm1 protein and the like to be excessively present in a brain of a mammal, and a non-human mammal, to which the method has been applied, and the like.

Method of ameliorating symptom caused by mood disturbance

A novel protein (Gm1) includes an amino acid sequence part having a high homology with a domain having a high homology with a GTP binding site and a GTPase site conserved among G protein &#x3b1; subunits and a trimer forming domain conserved among G protein &#x3b1; subunits. The Gm1 protein is involved in signal transduction via a G protein-coupled receptor (GPCR) stimulation. The Gm1 protein is expressed intensively in human brain, thymus, testes, spleen, small intestine, uterus and heart. A method for screening for a substance capable of regulating a cellular signal transduction employs a polynucleotide encoding the Gm1 protein.

Protein involved in G protein-coupled receptor mediated signal transduction with ability to mediate signal transduction from a dopamine D1 receptor or an adenosine A2A receptor to an adenylate cyclase and utilization thereof

The present invention provides a common marmoset-derived hypoxanthine-guanine phosphoribosyltransferase gene and use thereof.

Common marmoset-derived HPRT gene and use thereof

Raffinose synthase genes coding for proteins capable of producing raffinose by combining a D-galactosyl group through an &#x3b1;(1&#x2192;6) bond with a hydroxyl group attached to the carbon atom at position 6 of a D-glucose residue in a sucrose molecule were isolated from various plants. These raffinose synthase genes are useful to change the content of raffinose family oligosaccharides in plants.

Raffinose synthase genes and their use

The present invention provides a common marmoset-derived glyceraldehyde-3-phosphate dehydrogenase gene and use thereof.

Common marmoset-derived glyceraldehyde-3-phosphate dehydrogenase gene and use thereof

We identified a novel protein (Gm1) comprising an amino acid sequence part having a high homology with a domain having a high homology with a GTP binding site and a GTPase site conserved among G protein &#x3b1; subunits and a trimer forming domain conserved among G protein &#x3b1; subunits. The Gm1 is involved in an signal transduction via a G protein-coupled receptor (GPCR) stimulation. Accordingly, this protein is considered to be a novel G protein. The Gm1 is expressed intensively in human brain, thymus, testes, spleen, small intestine, uterus and heart. We also established a method for screening for a substance capable of regulating a cellular signal transduction employing a polynucleotide encoding the Gm1.

G proteins, polynucleotide encoding the same and utilization thereof

Promoters capable of efficiently expressing a gene of interest in plants include (1) either a DNA containing SEQ ID No:1 or SEQ ID No:7, or a biological functional equivalent DNA containing SEQ ID No:1 or SEQ ID No:7 that have modifications in their respective nucleotide sequence provided that each retains more than 90% identity to the nucleotide sequence of any region containing at least 250 bp within SEQ ID No:1 or SEQ ID No:7, respectively. Terminators capable of efficiently expressing a gene of interest in plants include either a DNA containing SEQ ID No:2, or a biological functional equivalent DNA containing a SEQ ID No:2 that has a modified nucleotide sequence provided that it retains more than 90% identity to the nucleotide sequence of any region containing at least 250 bp within SEQ ID No:2.

Plant promoters and plant terminators

The present invention provides a common marmoset-derived beta-actin gene and use thereof.

Common marmoset-derived beta-actin gene and use thereof

The present invention provides a promoter including the following DNA (a) or (b): (a) the DNA consisting of the nucleotide sequence of nucleotide numbers 1743 to 2301 in the nucleotide sequence represented by SEQ ID: No. 1; or, (b) the DNA which can be hybridized with said DNA (a) in a stringent condition and having a promoter function in a plant cell, and the like.

Plant promoters

There is provided a cytoplasmic male sterility DNA factor, a method of discrimination which enables confirmation of the cytoplasmic male sterility in a short time.

Cytoplasmic male sterility DNA factor and utilization thereof

The present invention provides a promoter which is characterized in that it has following DNA sequence (a) or (b) and a DNA having the nucleotide sequence shown by SEQ ID:No.1, (a) a DNA sequence having the nucleotide sequence represented by at least nucleotide numbers 112-246 within the nucleotide sequence shown by SEQ ID:No.2, (b) a DNA sequence having the nucleotide sequence represented by at least base numbers 186-282 within the nucleotide sequence shown by SEQ ID:No.3 (hereinafter referred to as the present promoter), a chimera gene having the above promoter, a vector having the above promoter, a transformant characterized in that the promoter, the chimera gene or the vector is introduced into a host cell, a method for expressing gene comprising a step of expressing the desired gene in a host cell under the control of the promoter, use of the promoter for controlling the expression of a desired gene in a host cell, and a method for producing the promoter. According to the present invention, there becomes possible to provide a compact promoter suitable for more highly expressing the desired gene in the particular tissue than in other tissues in a host.

Plant promoter

The present invention relates to a promoter functional in plant cells and plasmid that can regulate efficient expression of a gene of interest in plant cells. The promoter comprises a G-Box element, which enhances expression of an operably linked gene of interest in plants or plant cells. A further object of the invention is the plasmid pGbox10 or pGbox11, as well as plants or plant cells transformed with said plasmid.

Plant promoter comprising a G-box element, GCCACGTGCC or GCCACGTGAG, and an application thereof

There is disclosed a plant promoter which is functional in plant cells comprising a nucleotide sequence (about 250 bp) of SEQ ID NO: 1 and use thereof.

Growing community of inventors

Kyoto, Japan

Kenji Oeda