Arkray, Inc.

Kyoto University

Kenji Nagakawa

Konomu Hirao

Hiroyuki Kimura

Hideo Saji

Hideaki Yamaoka

Nobuya Inagaki

Kentaro Toyoda

Hirokazu Matsuda

Masaaki Teramoto

Susumu Nishino

Tomomichi Tsujimoto

Yoshiyuki Kawase

Koji Katsuki

Masashi Okamoto

Mitsuhiro Hoshijima

Yoshiaki Fujinawa

Yu Ogawa

Sadaaki Kimura

A molecular probe for imaging of pancreatic islets is provided. The molecular probe includes a polypeptide represented by the following formula (1), or a polypeptide that has a homology with the foregoing polypeptide. Wherein 'X' represents a lysine residue, an amino group of a side chain of the lysine residue being labeled with a group represented by the following chemical formula (I), wherein A represents an aromatic hydrocarbon group or an aromatic heterocyclic group; Rrepresents a substituent that containsC,N,O,F,Cu,Ga,Ga,Br,Br,Br,Tc,In,I,I,I, orI; Rrepresents either a hydrogen atom, or a substituent different from that represented by R; and Rrepresents any one of a bond, an alkylene group having 1 to 6 carbon atoms, and an oxyalkylene group having 1 to 6 carbon atoms.

Molecular probe for imaging of pancreatic islets and use of the same

The present invention relates to technology for constructing a reaction system including a test target, an oxidation-reduction enzyme, and an electron mediator, and measuring the concentration of the test target by an electrochemical process. A Ru compound is used as the electron mediator. The present invention provides a concentration test instrument including a substrate, first and second electrodes formed on the substrate, and a reagent layer formed as a solid. The reagent layer contains an oxidation-reduction enzyme and a Ru compound, and is constituted so as to dissolve and construct a liquid phase reaction system when a sample liquid is supplied.

Concentration measuring method, concentration test instrument, and concentration measuring apparatus

A precursor of a molecular probe for imaging of pancreatic islets is provided. The precursor includes a polypeptide represented by any one of the following formulae (1) to (12), or a polypeptide having a homology with the foregoing polypeptide: 

Molecular probe precursor for imaging of pancreatic islet, and use thereof

A molecular probe for use in imaging of pancreatic islets is provided. The molecular probe comprises a polypeptide represented by the following formula (1), (2), or (3), or a polypeptide having homology with the foregoing polypeptide, where, in the formulae (1) and (2), 'X' represents a lysine residue, an amino group of a side chain of the lysine residue being labeled with a radioactive nuclide, and 'Z&#x2014;' indicates that an &#x3b1;-amino group at an N-terminus is not modified, or is modified with a modifying group having no electric charge; in the formula (3), &#x201c;B&#x2014;&#x201d; indicates that an &#x3b1;-amino group at an N-terminus is labeled with a radioactive nuclide; and in the formulae (1), (2), and (3), &#x201c;&#x2014;NH&#x201d; indicates that a carboxyl group at a C-terminus is amidated.

The present invention relates to an enzyme electrode including: a carbon particle; a metal particle held on the carbon particle, the metal particle having a catalytic activity against a redox reaction; a redox enzyme. The enzyme electrode of the present invention further includes a high-resistance particle enhancing an electrical resistance, the high-resistance particle being chemically stable. The high-resistance particle contains an inorganic substance, for example. The inorganic substance is aluminum oxide or smectite, for example.

Enzyme electrode

To provide a molecular probe for imaging of pancreatic islets. A molecular probe for use in imaging of pancreatic islets is provided. The molecular probe includes any one of the following polypeptides: polypeptides represented by the following formulae (1), (5), and (9); and polypeptides having homology with the foregoing polypeptides: where X in the formulae (1) and (5) and B- in the formula (9) indicate that an amino group is labeled with a group represented by the formula (I) below having an aromatic ring, wherein A represents either an aromatic hydrocarbon group or an aromatic heterocyclic group, Rrepresents a substituent that contains radioactive iodine, Rrepresents either a hydrogen atom or a substituent different from that represented by R, and Rrepresents any one of a bond, a methylene group, and an oxymethylene group.

The present invention provides a colorimetric method that can perform a simple and reliable analysis in a short time. The method includes transferring an electron from an analyte to a coloring reagent that produces color by reduction via a mediator by using an oxidoreductase; and performing qualitative or quantitative analysis of the analyte by measuring color produced in the coloring reagent. The enzyme reaction of this method is a single stage reaction, and the color production reaction occurs via the mediator. Therefore, the measurement can be performed in a short time. Since this reaction requires neither hydrogen peroxide nor oxygen, the measured values are highly reliable.

Colorimetric method and reagent used for the same

The present invention relates to an analytical instrument () which includes a flow path () for moving a sample containing blood cells, an introduction port () for introducing the sample into the flow path (), a reagent portion () arranged in the flow path (), and an electron detection medium () for obtaining information necessary for analyzing an analysis target component contained in the sample in relation with an amount of electrons transferred. The reagent portion () contains an electron mediator for supplying an electron taken from the analysis target component in the sample to the electron detection medium (), and at least part of the reagent portion is positioned adjacent to the introduction port ().

Analytical instrument having improved arrangement of reagent section and analytical method

The present invention provides a calorimetric method that can perform a simple and reliable analysis in a short time. The method includes transferring an electron from an analyte to a coloring reagent that produces color by reduction via a mediator by using an oxidoreductase; and performing qualitative or quantitative analysis of the analyte by measuring color produced in the coloring reagent. The enzyme reaction of this method is a single stage reaction, and the color production reaction occurs via the mediator. Therefore, the measurement can be performed in a short time. Since this reaction requires neither hydrogen peroxide nor oxygen, the measured values are highly reliable.

Growing community of inventors

Kyoto, Japan

Kenji Nagakawa