Asahi Kasei Kabushiki Kaisha

Banyu Pharmaceutical Co. Ltd

Asahi Kasei Kogyo Kabushiki Kaisha

Kenji Matsuyama

Koji Maehana

Seiichi Tanaka

Hajime Morishima

Shugo Atsuumi

Masato Nakano

Yutaka Koike

Takamitsu Utsumi

Susumu Nakagawa

Takehiro Fukami

Kiyofumi Ishikawa

Kenji Niiyama

Takashi Hayama

Mitsuo Yano

Fumio Nakano

Kazuhito Noguchi

Masaaki Hosoi

Akio Matsuda

Hiroshi Takeshita

Katsuya Watanabe

Yutaka Kokie

Keizou Yamamoto

Shigeaki Ichikawa

Mariko Fujimura

The object is to provide a lysis method, lysis treatment solution, detection method using an immunochromatographic device, and detection kit comprising an immunochromatographic device for detecting whether causative bacteria of mastitis are coliform bacteria or not by using milk of a livestock animal. There is provided a method for lysing coliform bacteria, which comprises the step of mixing a lysis agent containing a lytic enzyme, and at least one kind of anionic surfactant, and preferably further containing at least one kind of nonionic surfactant, with milk obtained form a livestock animal to lyse coliform bacteria existing in the milk. The lytic enzyme is preferably lysozyme.

Method for detecting coliform bacteria contained in milk

The object is to provide a lysis method and lysis treatment solution for efficiently lysing cells of variousbacteria in milk of a livestock animal to release a specific antigen substance contained in the cells for detecting whether causative bacterium of mastitis is abacterium or not by using the milk, as well as a detection method using an immunochromatographic device. There is provided a method for lysing abacterium, which comprises the step of mixing a lysis agent containing a lytic enzyme with milk obtained form a livestock animal to lyse abacterium existing in the milk. The lytic enzyme is preferably at least one selected from the group consisting of lysozyme, labiase, and &#x3b2;-N-acetylglucosaminidase.

Method for detectingbacterium contained in milk

The object is to provide a lysis method, lysis treatment solution, detection method using an immunochromatographic device, and detection kit comprising an immunochromatographic device for detecting whether causative bacterium of mastitis is aor not by using milk of a livestock animal. There is provided a method for lysing a, which comprises the step of mixing a lysis agent containing a lytic enzyme, and at least one kind of ampholytic surfactant, and preferably further containing at least one kind of nonionic surfactant, with milk obtained form a livestock animal to lyse aexisting in the milk. The lytic enzyme is preferably lysostaphin.

Method for detectingcontained in milk

An immunochromatographic method for detecting a specific substance contained in milk, which comprises (1) the step of contacting the milk with a test strip having a first part retaining a labeled first antibody directed to the specific substance or the specific substance that is labeled, a second part disposed downstream from the first part, on which a second antibody directed to the specific substance is immobilized, and a third part disposed upstream from the first part or the second part and having voids enabling removal of milk fat globules contained in the milk, at the third part or a further upstream part, and (2) the step of flowing the milk up to the second part or a further downstream part to obtain a detectable signal of the label at the second part or a further downstream part.

Method for detecting specific substance in milk

The object is to provide a lysis method, lysis treatment solution, detection method using an immunochromatographic device, and detection kit comprising an immunochromatographic device for detecting whether causative bacterium of mastitis is aor not by using milk of a livestock animal. There is provided a method for lysing awhich comprises the step of mixing a lysis agent containing a lytic enzyme, and at least one kind of ampholytic surfactant, and preferably further containing at least one kind of nonionic surfactant, with milk obtained form a livestock animal to lyse aexisting in the milk. The lytic enzyme is preferably lysostaphin.

[object Object]

An N-acylamino acid derivative of the formula: ##STR1##

N-acylamino acid derivatives and their use

An N-acylamino acid derivative of the formula: ##STR1## wherein each of R.sup.1, R.sup.2, R.sup.4 and R.sup.6 is hydrogen lower alkyl, cycloalkyl, cycloalkylalkyl, aryl, aralkyl or a monocyclic or bicyclic heterocyclic group containing from to 4 hetero atoms; each of R.sup.3 and R.sup.5 is hydrogen or lower alkyl; A is --CH(OH)--(CH.sub.2).sub.q R.sup.7 wherein R.sup.7 is hydrogen, lower alkyl, cycloalkyl, cyloalkylalkyl, aryl, aralkyl, a monocyclic or bicyclic heterocyclic group containing from to 4 hereto atoms or --E--R.sup.10 wherein E is --S(O).sub.i -- wherein i is 0, 1 or 2, oxygen, --NR.sup.11 -- wherein R.sup.11 is hydrogen, lower alkyl, cycloalkyl, cycloalkylalkyl, aryl or aralkyl, or ##STR2## wherein each of R.sup.12 and R.sup.13 is hydrogen, lower alkyl cycloalkyl, cycloalkylalkyl, aryl or aralkyl, and R.sup.10 is hydrogen, lower alkyl, cycloalkyl, cycloalkylalkyl, aryl, aralkyl or a monocyclic or bicyclic heterocyclic group containing from 1 to 4 hetero atoms provided that when R.sup.10 is hydrogen, i is 0, and a is an integer of from 0 to 5; or --CH.sub.2 --CHR.sup.8 --CO--R.sup.9 wherein R.sup.8 is hydrogen, lower alkyl, cycloalkyl, cycloalkylalkyl, aryl, aralkyl or a monocyclic or bicyclic heterocyclic group containing from 1 to 4 hetero atoms, and R.sup.9 is hydroxyl, --OX wherein X is alkyl, aryl, lower alkoxycarbonyloxyalkyl or 1-phthalidyl, or --N(Y.sup.1) (Y.sup.2) wherein each of Y.sup.1 and Y.sup.2 is hydrogen, lower alkyl, aryl, aralkyl or cycloalkyl, or Y and.sup.1 and Y.sup.2 form together with the adjacent nitrogen atom a 5- or 6-membered heterocyclic group which may contain a further hereto atom; m is 0, 1 or 2; and n is an integer of from 1 to 5, provided that when R.sup.1 is hydrogen, m is 0; or a salt thereof, which is useful as hypotensive drugs.

A DNA is disclosed, which comprises a gene coding for deacetylcephalosporin C acetyltransferase, i.e., an enzyme capable of catalyzing the final step of the biosynthesis of cephalosporin C. The gene may be either a cDNA or a genomic DNA. Also disclosed is a recombinant DNA comprising a vector having inserted therein at least a portion of the above-mentioned DNA, which recombinant DNA is in a form such that the portion is capable of expression in Acremonium chrysogenum. Acremonium chrysogenum transformed with the recombinant DNA can be advantageously used for efficiently preparing cephalosporin C.

Deacetylcephalosporin C acetyltransferase from Acremonium chrysogenum

The present invention provides an N-substituted acylamino acid compound of the formula: ##STR1## a process for their production and pharmaceutical uses, and intermediates useful for their production.

N-substituted acylamino acid compounds, process for their production and

There are provided novel aminophosphonic acid derivatives of the general formula [I]: ##STR1## wherein n is an integer of 2 or 3; R.sup.1 is a phenyl, naphthyl, indolyl, benzothienyl, benzofuryl or benzoxazolyl group which may have 1 to 3 substituents selected from the group consisting of hydroxyl and lower alkyloxy groups, or a hydrogen atom; R.sup.2 is a lower alkyl group, a benzyl group, a 4-hydroxybenzyl group, a 3-indolylmethyl group or a .beta.-phenethyl group; and A is an optional residue of an amino acid selected from the group consisting of tryptophane, tyrosine, phenylalanine, homophenylalanine, naphthylalanine or N.sup..omega. -nitroarginine, and their pharmaceutically acceptable salts. The compounds according to the present invention and their pharmaceutically acceptable salts exhibit an inhibitory activity against endothelin-converting enzyme, and are expected to be useful as a drug treating various diseases with which endothelin is concerned.

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Tokyo, Japan

Kenji Matsuyama