Nippon Oil Company

Kenji Maruhashi

Ichiro Kojima

Yasuo Fujiwara

Tetsuo Satoh

Yutaka Oguchi

Noboru Endoh

Tatsuo Kinoshita

A process for separating porphyrins, which comprises

Process for separating porphyrins

A process for separating vitamin B.sub.12 in pure form, which comprises bringing a vitamin B.sub.12 -containing liquid containing impurities into contact with a resin selected from the group consisting of (A) a divinylbenzene/styrene copolymer having a mode pore diameter of at least about 200 A and a total pore volume of more than 0.6 ml/g, and (B) a copolymer of divinylbenzene/styrene/an unsaturated alkyl ester of an aromatic polycarboxylic acid represented by the formula ##STR1## wherein R represents a C.sub.3 -C.sub.10 unsaturated alkyl moiety having a carbon-carbon double bond and n is 2 or 3, said copolymer (B) having a surface area of at least about 700 m.sup.2 /g, to cause vitamin B.sub.12 to be adsorbed to said resin, and eluting the vitamin B.sub.12 with an eluent, and collecting the active eluate.

Process for purifying and separating vitamin B.sub.12

A process for producing uroporphyrin III, which comprises cultivating a uroporphyrin III-producing microorganism of the genus Arthrobacter in a culture medium containing a carbon source, a nitrogen source and a mineral, and recovering uroporphyrin III from the culture broth.

Process for producing uroporphyrin III

A process for separating and recovering coproporphyrin and uroporphyrin from a culture broth containing them, which comprises

Process for separating and recovering coproporphyrin and uroporphyrin

In a process for producing coproporphyrin III which comprises cultivating a coproporphyrin III-producing microorganism of the genus Arthrobacter in a culture medium containing a carbon source, a nitrogen source and a mineral and recovering coproporphyrin III from the culture broth, the improvement wherein the culture medium contains at least 0.1 g, per liter of the culture medium, of L-cystine, at leas 0.5 g, per liter of the culture medium, of Mg.sup.++, or both; and coproporphyrin III-producing microoganisms.

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Yokohama, Japan

Kenji Maruhashi