Toray Industries, Inc.

Kenji Kawamura

Katsushige Yamada

Masateru Ito

Kyohei Isobe

Satoshi Sakami

Izumi Morita

Hideki Sawai

Daijiro Tsukamoto

Kenji Sawai

Takashi Mimitsuka

Shin-ichi Minegishi

Shinichi Minegishi

Tatsuya Nagano

Masato Akahira

Izumi Nakagawa

Miyuki Horiguchi

Mark Joseph Burk

Tetsuya Yamada

Koji Yamauchi

Sadanori Kumazawa

Michael Japs

Daisuke Yamamoto

Kohei Yamashita

Warren Clark

Hideshi Yanase

Shinichi Yamane

Ito Masateru

Masataka Makino

Shin-Ichi Minegishi

The present invention relates to a method of producing ε-caprolactam, the method including the following steps (A) and (B): (A) a step of reacting 5-cyanovaleramide with hydrogen in an aqueous solvent in a presence of a hydrogenation catalyst to obtain a 5-cyanovaleramide hydrogenation reaction mixture; (B) a step of heating the 5-cyanovaleramide hydrogenation reaction mixture at a temperature of 180° C. or higher and 300° C. or lower in an aqueous solvent to obtain ε-caprolactam.

Method for producing ε-caprolactam

Disclosed is a genetically modified microorganism into which a nucleic acid encoding an enzyme that catalyzes a reaction to reduce 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA is introduced. In the genetically modified microorganism, a nucleic acid encoding any one of the polypeptides described in (a) to (c) below is introduced or expression of the polypeptide is enhanced: (a) a polypeptide composed of an amino acid sequence represented by any one of SEQ ID NOs: 1 to 6 and 213, (b) a polypeptide composed of the same amino acid sequence as any one of the amino acid sequences, except that one or several amino acids are substituted, deleted, inserted, and/or added, and having enzymatic activity that catalyzes a reaction to reduce 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA, and (c) a polypeptide composed of an amino acid sequence with a sequence identity of not less than 70% to any one of the amino acid sequences and having activity to reduce 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA.

Gene-modified microorganism for producing 3-hydroxyadipic acid, alpha-hydromuconic acid, and/or adipic acid, and production method for said chemical products

A method relates to producing 3-hydroxyadipic acid-3,6-lactone, the method including the following steps (A) and (B): (A) a step of adding an acid to a 3-hydroxyadipic acid-containing aqueous solution to obtain a 3-hydroxyadipic acid-3,6-lactone-containing aqueous solution; and (B) a step of obtaining a 3-hydroxyadipic acid-3,6-lactone extract by bringing the 3-hydroxyadipic acid-3,6-lactone-containing aqueous solution obtained in step (A) into contact with an extraction solvent that is phase-separated from the solution.

Method of producing 3-hydroxyadipic acid-3,6-lactone

A method of producing 3-oxoadipic acid from an aliphatic compound easily utilizable by a microorganism, such as a saccharide, by utilization of a metabolic pathway of the microorganism is disclosed. The method of producing 3-oxoadipic acid includes the step of culturing at least one type of microorganism having a capacity to produce 3-oxoadipic acid, selected from the group consisting of, for example, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, and microorganisms belonging to the genus

Method for producing 3-Oxoadipic acid

Lactic acid is obtained by a method including (A) a step of continuous fermentation wherein a fermentation culture medium of a microorganism having an ability of lactic acid fermentation is filtered through a porous membrane having an average pore size of not less than 0.01 &#x3bc;m and less than 1 &#x3bc;m with a transmembrane pressure difference within the range of 0.1 to 20 kPa, and the permeate is collected, while retaining the non-permeated liquid in or returning the non-permeated liquid to the culture, and adding a fermentation feedstock to the culture; (B) a step of filtering the permeate obtained in Step (A) through a nanofiltration membrane; and (C) a step of distilling the permeate obtained in Step (B) under a pressure of not less than 1 Pa and not more than atmospheric pressure, at 25&#xb0; C. to 200&#xb0; C. to recover lactic acid.

Method for producing lactic acid and method for producing polylactic acid

Provided is a method of producing &#x3b1;-hydromuconic acid, the method including the step of culturing a microorganism belonging to the genuscapable of producing &#x3b1;-hydromuconic acid.

Method for producing alpha-hydromuconic acid

A method of producing &#x3b1;-hydromuconic acid using a metabolic pathway of a microorganism is disclosed. The method of producing &#x3b1;-hydromuconic acid includes the step of culturing at least one type of microorganism having a capacity to produce &#x3b1;-hydromuconic acid, selected from the group consisting of microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, and microorganisms belonging to the genus

Method for producing &#x3b1;-hydromuconic acid

Provided is a method of producing 3-hydroxyadipic acid, the method including the step of culturing a microorganism belonging to the genuscapable of producing 3-hydroxyadipic acid.

Method for producing 3-hydroxyadipic acid

A novel method of producing 3-hydroxyadipic acid using a metabolic pathway of a microorganism is disclosed. The method of producing 3-hydroxyadipic acid includes the step of culturing at least one type of microorganism having a capacity to produce 3-hydroxyadipic acid, selected from the group consisting of microorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusand microorganisms belonging to the genus

Method for producing 3-oxoadipic acid

A diol composition includes a diol as a major component and has an electrical conductivity of 0.6 to 30 mS/m.

Diol composition and polyester

A method of producing lactic acid includes filtering a lactic acid-containing aqueous solution through a nanofiltration membrane to recover an aqueous lactic acid solution from the permeate side (Step A); distilling the aqueous lactic acid solution to recover lactic acid from the vapor side (Step B); and crystallizing the lactic acid obtain in Step B, and performing solid-liquid separation to recover a lactic acid crystal(s) (Step C).

Method of producing lactic acid and polylactic acid

A method of producing 2,3-butanediol includes subjecting a 2,3-butanediol culture liquid produced by microbial fermentation to nanofiltration membrane treatment and ion-exchange treatment (Step A), and then adding an alkaline substance and performing distillation (Step B).

Process for producing 2,3-butanediol

A method of producing lactic acid includes subjecting a lactic acid-containing solution to distillation to collect lactic acid from the vapor side (Step A); subjecting the lactic acid obtained in Step A to crystallization (Step B); subjecting the lactic acid slurry obtained in Step B to solid-liquid separation into lactic acid crystals and a mother liquor (Step C); and circulating the mother liquor obtained in Step C to Step B (Step D).

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Kamakura, Japan

Kenji Kawamura