Ikeda Food Research Co., Ltd.

Phc Corporation

Sony Corporation

Toyota Jidosha Kabushiki Kaisha

Kyoto University

Kanazawa University

National University Corporation Kanazawa University

Kenji Kano

Atsushi Sato

Tokuji Ikeda

Seiya Tsujimura

Takako Yada

Hironori Omura

Hirokazu Sanada

Tetsunari Morita

Mika Kuyama

Hisao Kato

Norihiko Setoyama

Tsutomu Kajino

Takeshi Sakurai

Kunishige Kataoka

Keiko Uemura

Mika c/o Ikeda Food Research Co Ltd Kuyama

Hirokazu c/o Ikeda Food Research Co Ltd Sanada

Takako c/o Ikeda Food Research Co Ltd Yada

Hironori c/o Ikeda Food Research Co Ltd Omura

Tetsunari c/o Ikeda Food Research Co Ltd Morita

The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.

Coenzyme-binding glucose dehydrogenase

The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase, According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.

An immobilization carrier containing an electron acceptor compound is used in addition to glutaraldehyde and poly-L-lysine to immobilize an enzyme and an electron acceptor compound simultaneously to an electrode. For example, here are used diaphorase as the enzyme and 2-amino-3-carboxy-1,4-naphthoquinone (ACNQ) as the electron acceptor compound.

Immobilization support, process for producing the same, electrode, process for producing the same, electrode reaction utilizing apparatus and process for producing the same

A novel electrocatalyst made of an oxidase having high electron transfer efficiency and an enzymatic electrode using the same are provided. The electrocatalyst is made of CueO. The enzymatic electrode comprises a carbonaceous porous body and an electrocatalyst made of CueO supported on the surface of the carbonaceous porous body. CueO is preferably CueO from. The carbonaceous porous body constituting the enzymatic electrode is preferably carbonaceous gel. Also, the enzymatic electrode may further comprise a mediator which facilitates transfer of electron between the carbonaceous porous body and said CueO.

Electrocatalyst and enzymatic electrode

An immobilization carrier containing an electron acceptor compound is used in addition to glutaraldehyde and poly-L-lysine to immobilize an enzyme and an electron acceptor compound simultaneously to an electrode. For example, here are used diaphorase as the enzyme and 2-amino-3-carboxy-1, 4-naphthoquinone (ACNQ) as the electron acceptor compound.

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Kyoto, Japan

Kenji Kano