Shionogi Company Limited

Toyo Boseki Kabushiki Kaisha

Yamasa Corporation

Kenji Inagaki

Hidehiko Tanaka

Kenji Soda

Nobuyoshi Esaki

Hiroyuki Inoue

Manabu Sugimoto

Atsushi Sogabe

Masanori Oka

Akio Takimoto

Makoto Ashiuchi

Takashi Hatta

Tomoaki Takakura

Hitoshi Kusakabe

Shintaro Misaki

Jiro Arima

Hiroshi Nishise

Toshiharu Yagi

The disclosure relates to (i) a gene isolated fromsp.JCM2471, the gene encoding a new glycerol kinase and (ii) a method for preparing the glycerol kinase by gene recombination technique, A Glycerol kinase which has high resistance against preservative, a recombinant vector comprising a gene encoding the glycerol kinase, a transformant prepared by transforming a host cell with the recombinant vector, and a method for producing the glycerol kinase, including culturing the transformant to produce glycerol kinase, and collecting the resulting glycerol kinase.

Glycerol kinase, which has high resistance against preservative

An L-methionine &#x3b3;-lyase having modified function which is obtained by structurally stabilizing natural L-methionine &#x3b3;-lyase by analyzing the structure of crystals thereof by using X-ray, estimating an amino acid sequence concerning its substrate specificity and varying amino acid residue thereof; enzymes comprising a protein constituting the L-methionine &#x3b3;-lyase having modified function or its multimer (preferably its tetramer); DNA sequences encoding these enzymes; a process for producing these enzymes; and medicinal preparations (preferably anticancer agent) containing these enzymes.

L-methionine $g(g)-lyase with modified function

The present invention provides a novel L-glutamate oxidase, a gene encoding the enzyme, and a method for producing the enzyme. By use of a gene encoding the enzyme, L-glutamate oxidase can be readily prepared at low costs through a recombinant DNA technique. The novel L-glutamate oxidase has the following physicochemical properties:

L-glutamate oxidase

A recombinant protein capable of constituting a recombinant L-methionine .gamma.-lyase or a variant thereof, a recombinant oligomeric enzyme, preferably tetrameric enzyme, consisting of the recombinant protein, a DNA molecule encoding the same, an expression vector containing the DNA molecule, a host cell harboring the expression vector, a method of preparing the recombinant L-methionine .gamma.-lyase, and anti-tumor agent comprising the recombinant L-methionine .gamma.-lyase or a variant thereof are provided.

Recombinant L-methionine Y-lyase

Recombinant L-methionine .gamma.-lyase

Growing community of inventors

Okayama, Japan

Kenji Inagaki