Yokogawa Electric Corporation

Kenji Hachiya

Takayuki Kei

Kenta Mikuriya

Yasuhiro Tanibata

A method for adjusting a confocal microscope which includes a microscope unit and a confocal scanner unit of Nipkow disk type, emits a light beam for image measurement on a sample, images a returned fluorescent light of the light beam by a CCD (charge-coupled device) digital camera as a confocal image, and enables observation of the sample by the confocal image, the method includes matching a phase of rotation of the Nipkow disk with a phase of an exposure time of the CCD digital camera.

Method for adjusting confocal microscope

The present invention is characterized in that it is possible to provide a three-dimensional confocal microscope system configured so that an objective lens can be scanned in the optical-axis direction using an actuator to obtain sliced confocal images of a sample, wherein the actuator is driven using a scanning waveform signal, which is triangular or step-like and has been corrected so that acceleration is kept virtually constant at discontinuous points of change in the scanning waveform signal, thereby consistently providing sliced confocal images of the sample.

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Musashino, Japan

Kenji Hachiya