Fuji Photo Film Company, Limited

Fuju Photo Film Co., Ltd.

Kenichiro Yazawa

Masao Kitajima

Osamu Seshimoto

Asaji Kondo

Takaki Arai

Fumio Sugaya

Eiichi Hasegawa

Shigeru Tezuka

Shizuo Miyano

Shinzo Kishimoto

Fuminori Arai

Shunichi Seto

Toshihisa Ito

Akemi Higo

Kiyotaka Fujiwara

Harumi Katsuyama

Masataka Murata

Isamu Hatanaka

Hideaki Takeuchi

Sigeru Tezuka

Yasuo Washizawa

Takeshi Igarashi

Keiichi Ishizaki

Yohonosuke Takahashi

Shigeo Tanaka

Takashi Sekine

Takasi Sekine

This invention provides a continuous blood filtration apparatus capable of filtering a plurality of blood samples continuous by using blood filter units to prepare plasma or serum samples in a short period, which comprises, a conveyor conveying a plurality of blood reservoirs each of which contains a blood sample and in which a suction nozzle of a blood filter unit has been put, a manifold connected to a suction line, couples of a valve and a connector for connecting the manifold to the blood filter unit provided on the end of each branch of the manifold, and a mechanism of moving the blood reservoirs in vertical direction.

Continuous blood filtration apparatus

A method for preparing a plasma or serum sample from a whole blood sample, by contacting a glass fiber filter which contains calcium, sodium or potassium components with an aqueous solution of an organic acid or a mineral acid to elute the calcium, sodium and potassium components in the glass fiber filter into the aqueous solution, separating the aqueous solution from the filter and washing the glass fiber filter with water to remove any residual aqueous solution adhered thereto and then filtering a whole blood sample through the thus treated fiber filter.

Method of preparing glass fiber filter

The blood filter cartridge which can collect a minor component of blood efficiently in a high concentration comprises a blood filtering material and a holder containing the blood filtering material and having a blood inlet and a filtrate outlet, wherein an antibody or an antigen is immobilized on at least a part from the blood inlet to the filtrate outlet or a filtrate receiver.

Blood filter cartridge

Method of analyzing blood component

The invention provides a method of recovering filtration residues of blood which comprises filtering blood by a blood filtering device comprising a blood filtering material which is a depth filtering material and a holder which contains the blood filtering material and has a blood inlet and filtrate outlet, after finishing the filtering blood, feeding aqueous liquid to the filtrate outlet to recover the filtration residues accumulated in the blood filtering material.

Method of recovering blood filtration residues

The blood filter cartridge can filter almost total volume of drawn blood efficiently which comprises a blood filtering material and a holder containing the blood filtering material, wherein the holder is provided with a blood reservoir which connects with an inflow side of the blood filtering material and a filtrate receiver which connects with an outflow side of the blood filtering material.

The blood filter cartridge of the invention can be used as a sample cup of an analyzer without sucking bubbles, which comprises a blood filtering material, a holder containing the blood filtering material and having a blood inlet and a filtrate outlet, and a filtrate receiver for receiving filtrate discharged from the filtrate outlet, the filtrate receiver has an upper end having a difference in height or structure for returning the filtrate from the upper end of the filtrate receiver to the filtrate outlet.

Blood filter cartridge with overflow receiver

This invention provides a blood filter unit capable of obtaining a necessary volume of a plasma or serum sample from blood to be assayed easily at any place, which comprises a blood filtering material and a holder accommodating the blood filtering material and having a nozzle for sucking blood and a filtrate outlet, and the inside of the holder being kept reduced pressure conditions.

Blood filter unit

The invention provides a blood filter cartridge which is made so that the filtrate discharged from the filtrate outlet is not drawn back to the inside of the filtering chamber. The blood filter cartridge includes a blood filtering material, a holder containing the blood filtering material and having a blood inlet and a filtrate outlet, and a filtrate receiver which receives the filtrate discharged from the filtrate outlet. The blood filter catridge is provided with a means for preventing the filtrate in the filtrate receiver from returning to inside of the holder upon finishing filtration. Such means include the following: removing opposite faces around the filtrate outlet, making length of lower end of the filtrate outlet 0.8 mm or more, smoothing surface around the filtrate outlet, and forming surface around the filtrate outlet by a material having a small surface energy.

The blood filter unit of the invention is composed of a blood filtering material made of glass fiber filter and microporous membrane and a holder having a blood inlet and a filtrate outlet. The holder accommodates the blood filtering material so that the microporous membrane is located on the filtrate outlet side, provides a space between the blood filtering material and the filtrate outlet, and provides a means for preventing adhesion of the blood filtering material on the filtrate outlet side. By using the blood filter unit, a necessary volume of plasma or serum for analysis can be separated surely, irrespective of hematocrit value of the blood.

This invention provides a method of filtering blood using a filtering material comprising glass fiber filter, capable of obtaining a desired amount of plasma or serum without hemolysis and leakage of blood cells easily which comprises keeping pressure difference between blood inlet side and filtrate outlet side 50 mmHg or less at least for 5 seconds from contacting the blood to be filtered, and keeping the pressure difference 200 mmHg or less throughout the filtering of the blood.

Method of filtering blood

A blood filter unit having a blood filter holder containing a blood filtering material of a glass fiber filter and a microporous membrane is described. The holder has a blood inlet and a filtrate outlet, and accommodates the blood filtering material so that the microporous membrane is located on the filtrate outlet side and has a space between the blood filtering material and the filtrate outlet. The holder provides means for preventing adhesion of the blood filtering material on the filtrate outlet side. The inventive blood filter unit allows separation of a desired volume of plasma or serum for analysis without interference by the hematocrit value of the blood.

The method of the invention is for preparing a plasma or serum sample from whole blood and includes mixing an aqueous solution of an inorganic salt or an amino acid or salt thereof with the whole blood in an amount of 20% or less of the whole blood volume so that concentration of the inorganic salt or the amino acid or salt thereof becomes 10 to 200 .mu.mol/1 ml whole blood, and then, filtering the whole blood to remove blood cell components. According to the method of the invention, plasma separation from whole blood can be achieved efficiently, and recovered volume of plasma can be increased. By selecting reagent, good plasma which has no deviation in component viewpoint, and which is possible to measure in the same level accuracy as a plasma obtained by centurifuging, can be obtained through a simple filtration operation.

Preparation of plasma or serum sample from whole blood

A spotting tip includes a tip body which is fitted on the end of a nozzle for sucking liquid contained in a container and inserted into the liquid in the container through a mouth of the container, and a lid member which is brought into contact with the mouth of the container and closes the space between the tip and the edge of the mouth when the tip is inserted into the container to a predetermined depth. At least one fine communicating passage is formed in at least one of the lid member and the tip body to communicate the inside and outside spaces of the container on opposite sides of the lid member.

Spotting tip

When determining an activity of particular predetermined ions in a sample liquid by use of a device for measuring ionic activity including a pair of ion-selective electrodes which are selectively responsive to particular predetermined ions, a pair of spotting holes through which a sample liquid and a reference liquid are respectively supplied to the ion-selective electrodes and a porous bridge which communicates the spotting holes with each other so as to permit the sample liquid and the reference liquid spotted to the spotting holes to electrically contact with each other, for instance, the sample liquid is first spotted to one of the spotting holes of the device for measuring ionic activity with a first spotting tool, and at least one of the first spotting tool and the device for measuring ionic activity is thereafter moved to remove the first spotting tool away from the device for measuring ionic activity. Then the reference liquid is spotted to the other spotting hole of the device for measuring ionic activity with a second spotting tool within a predetermined time after the sample liquid is spotted to the spotting hole.

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Saitama, Japan

Kenichiro Yazawa