Takeda Chemical Industries, Inc.

Kenichiro Miyagawa

Naoyuki Kanzaki

Muneharu Doi

Yukihiro Kanegae

Yoshio Sugiyama

Hideki Kizawa

Shun-ichi Akiyama

Junichi Miyazaki

A microorganism belonging to the Gram-positive cocci having not less than about 55 mol % of guanine plus cytosine content of DNA is cultivated in a medium. Trehalose is produced and extracellularly accumulated in the medium with a good yield. The present method, therefore, brings about a feasible method for the industrial production of trehalose. Preferably, the microorganism used is Deinococcus proteolyticus (IFO 15345), Deinococcus erythromyxa (IFO 15344), Deinococcus radiopugnans (IFO 15348), Micrococcus varians (FERM BP-4238 and IFO 3765), Micrococcus agilis (IFO 15323) or Microccus luteus (IFO 3067 and IFO 12708).

Method for the production of trehalose using strains of Micrococcus and

Disclosed are (1) a method of producing D-ribose which comprises cultivating a microorganism belonging to the genus Bacillus having D-ribose producing ability in a medium, the microorganism belonging to the genus Bacillus containing a DNA sequence participating in expression of a gluconate operon which is partly or wholly modified so as to highly express the gluconate operon in the microorganism belonging to the genus Bacillus, accumulating D-ribose, and collecting D-ribose thus obtained; (2) a novel microorganism belonging to the genus Bacillus having D-ribose producing ability transformed with DNA which contains a DNA sequence participating in expression of a gluconate operon which is partly or wholly modified so as to highly express the gluconate operon in the microorganism belonging to the genus Bacillus; (3) novel DNA in which a promoter of a gluconate operon of a microorganism belonging to the genus Bacillus is modified so as to highly express said gluconate operon in the microorganism belonging to the genus Bacillus; and (4) a novel vector into which DNA is introduced in which a promoter of a gluconate operon of a microorganism belonging to the genus Bacillus is modified so as to highly express the gluconate operon in the microorganism belonging to the genus Bacillus, whereby D-ribose can be produced in stable form in large amounts.

Host and vector for producing D-ribose

Method of producing inosine and/or guanosine by culturing an inosine and/or guanosine-producing mutant of the genus Bacillus which requires adenine for growth and is resistant to an antifolate. Thus, inosine and/or guanosine can be produced in much greater yields, compared with known methods.

Method of producing inosine and/or guanosine

Growing community of inventors

Osaka, Japan

Kenichiro Miyagawa