Toyo Kohan Co., Ltd.

Kenichi Takagi

Michifumi Tanga

Yoshihiko Isobe

Masao Komai

Hiroshi Okamura

Mitsuyoshi Ohba

Kaoru Yamakawa

It is intended to provide a method whereby DNA can be conveniently and rigidly immobilized on a substrate at such a high density as achieved by the conventional methods and a method of accurately detecting DNA. A substrate activation kit comprising a phosphate buffer (pH6), a solution A (an aqueous solution) of N-hydroxysuccinimide and a solution B (a dioxide solution) of 1-[3-(dimethylamino) propyl]3-ethylcarbodiimide; and a method of detecting DNA characterized by comprising spotting DNA on a substrate having been activated by using the above activation kit and then hybridizing the spotted DNA with fluorescence labeled DNA.

Substrate activation kit and method for detecting DNA and the like using the same

A heat-resistant sintered hard alloy comprises 35% to 95% by weight of a WCoB type complex boride in a cobalt base alloy. The alloy contains 1.5% to 4.1% boron, 19.1% to 69.7% tungsten, optionally to 25% chromium, the balance being cobalt and a maximum of 1% impurities. Nickel, iron and/or copper may be substituted for portions of the cobalt content.

Heat resistant sintered hard alloy

Growing community of inventors

Yamaguchi-ken, Japan

Kenichi Takagi