National Institute of Advanced Industrial Science and Technology

Eisai R&d Management Co., Ltd.

Neo-morgan Laboratory Incorporated

Kenichi Nakayama

Yasunori Chiba

Yoshifumi Jigami

Hiroko Abe

Yoshihiro Nakajima

Kappei Tsukahara

Mamiko Tsuchiya

Shohei Yamamura

Masatoshi Kataoka

Shouki Yatsushiro

Mariko Umemura

Yasuko Fujita

Akiko Itadani

Tomoko Iwaki

Michiyo Okamoto

Kazuya Tomimoto

Yuki Takaoka

The present invention provides: genetically modified yeasts such as mutant yeasts having an ability to produce N-linked sugar chains of ManGlcNAcand a decreased ability to produce O-linked sugar chains, mutant yeasts having an ability to produce N-linked sugar chains of ManGlcNAcand further having an ability to produce N-linked sugar chains of GlcNAcManGlcNAc, and mutant yeasts having an increased ability to produce and secrete proteins and an ability to produce N-linked sugar chains of ManGlcNAc; and a method for producing glycoproteins using them.

Sugar-chain modified yeast and method for producing glycoprotein using the same

The present invention enables screening for compounds that inhibit the transport of GPI-anchored proteins to fungal cell walls, using a simple assay for transacylation to GlcN-PI using membrane fraction expressing GWT1 protein. New antifungal agents can be created that inhibit the synthesis of fungal cell walls and also inhibit adhesion to host cells by inhibiting the transport of GPI-anchored proteins to fungal cell walls.

Method of screening for compounds that inhibit the enzymatic activity of GWT1 gene product

A method for breeding yeast having thermotolerance or recovering growth activity and a method for breeding yeast which produces beta-glucan efficiently as well as an yeast obtained by such methods for breeding are presented by a method for breeding yeast having thermotolerance or recovering growth activity including a step for controlling proofreading function of DNA polymerase in a loss-of-function mutant of yeast (for example, a step for including mutant pol3 gene or mutant cdc6&#x2212; gene in a gene-disruptant.

Breeding method for yeast, yeast and a production method for glycoprotein or beta-glucan

Growing community of inventors

Tsukuba, Japan

Kenichi Nakayama