Kabushiki Kaisha Dnaform

Nippon Steel Engineering Co., Ltd.

Kengo Usui

Yoshihide Hayashizaki

Yuki Tanaka

Yuki Kawai

Yasumasa Mitani

Yasumasa Kimura

Takahiro Arakawa

Masayoshi Itoh

Saori Goda

Kazuhito Nomura

Sotaro Uemura

Shoji Furutachi

The present invention is to provide a method for analyzing a target nucleic acid, by which the target nucleic acid can be analyzed rapidly and easily. In order to achieve the above object, the present invention provides a method for analyzing a target nucleic acid in a sample, including the step of: analyzing the target nucleic acid in the sample by bringing the sample into contact with a label and with a primer or probe that can hybridize to the target nucleic acid. The primer or probe is immobilized on a solid phase. The label does not emit light when the primer or probe does not hybridize to the target nucleic acid, whereas the label emits light when the primer or probe has hybridized to the target nucleic acid. The analysis is carried out by detecting the light emitted from the label.

Method for analyzing target nucleic acid, kit, and analyzer

The present invention provides a primer set including primers that can be designed easily, with which an amplification distance can be shortened. Provided is a primer set for use in a method for isothermally amplifying a target nucleic acid sequence 4. The primer set includes a first primer 1F and a second primer 1R. The first primer 1F includes, on the 3' side thereof, a sequence (A&#x2032;) that can hybridize to a sequence (A) on the 3&#x2032; side of the target nucleic acid sequence. The second primer 1R includes, on the 3&#x2032; side thereof, a sequence (B&#x2032;) that can hybridize to a sequence (B) on the 3&#x2032; side of either a strand extended from the first primer or a complementary strand of the target nucleic acid sequence 4. The first primer 1F and the second primer 1R include, on the 5&#x2032; sides thereof, sequences (C) that are substantially identical to each other.

Primer set for isothermal amplication of a target nucleic acid sequence

The present invention is to provide a pretreatment method that allows RNA to be detected promptly and simply. RNA degradation activity due to lactoferrin present in the human rhinal mucosa is inhibited, for example, by adding iron ion and carbonate ion to a biological sample that contains the human rhinal mucosa. With the pretreated biological sample, an RNA virus gene can be amplified by a reverse transcriptase. Iron ion and carbonate ion can also inhibit reverse transcriptase inhibition due to lysozyme C contained in the human rhinal mucosa. Further, it is preferable to remove the envelope of the RNA virus by adding SDS to the biological sample that contains the human rhinal mucosa.

Pretreatment method of biological sample, detection method of RNA, and pretreatment kit

Growing community of inventors

Yokohama, Japan

Kengo Usui