Mycogen Corporation

Kendrick Akira Uyeda

Jewel M Payne

Kenneth Edwin Narva

Tracy Ellis Michaels

Christine Julie Stalder

Gregory A Bradfisch

George E Schwab

August J Sick

Luis Foncerrada

Disclosed claimed are toxins produced by novel isolates designated . PS92J, . PS196S1, . PS201L1, and . PS201T6, which have dipteran and/or corn rootworm activity. Thus, the isolates, or mutants therof, can be used to control such pests. Further, claimed are novel genes encoding these &dgr;-endotoxins, which can be expressed in other hosts. Expression of the &dgr;-endotoxins in such hosts results in the control of susceptible insect pests in the environment of such hosts.

Bacillus thuringiensis isolates and toxins

The subject disclosure concerns,strains which can be used to control lepidopteran pests. The strains were previously known to control coleopteran pests. The discovery of lepidopteran activity was totally unexpected. These B.t. strains can be formulated using standard lepidopteran formulation procedures. Means of administration are also standard. The genes encoding lepidopteran-active toxins can be isolated from the B.t. isolates and used to transform other microbes or plants for use to control lepidopteran pests.

Process for controlling lepidopteron pests

Disclosed and claimed are approximately 130 kDa toxins produced by Bacillus thuringiensis PS201T6, which can be used to control pests. Further, claimed are novel genes encoding these .delta.-endotoxins, which can be expressed in other hosts. Expression of the .delta.-endotoxins in such hosts results in the control of susceptible insect pests in the environment of such hosts results in the control of susceptible insect pests in the environment of such hosts.

Polynucleotides encoding a 130 kDa pesticidal protein from Bacillus

Disclosed and claimed are toxins produced by novel Bacillus thuringiensis insolates designated B.t. PS92J, B.t. PS196S1, B.t. PS201L1; and B.t. PS201T6, which have dipteran and/or corn rootworm activity. Thus, the insolates, or mutants thereof, can be used to control such pests. Further, claimed are novel genes encoding these .delta.-emdotoxins, which can be expressed in other hosts, Expression of the .delta.-endotoxins in such hosts results in the control of susceptible insect pests in the environment of such hosts.

Disclosed and claimed are toxins produced by novel Bacillus thuringiensis isolates designated B.t. PS92J, B.t. PS196S1, B.t. PS201L1, and B.t. PS201T6, which have dipteran and/or corn rootworm activity. Thus, the isolates, or mutants thereof, can be used to control such pests. Further, claimed are novel genes encoding these .delta.-endotoxins, which can be expressed in other hosts. Expression of the .delta.-endotoxins in such hosts results in the control of susceptible insect pests in the environment of such hosts.

Bacillus thuringiensisisolate PS201T6 toxin

Bacillus thuringiensis cultures deposited as NRRL B-18747, NRRL B-18749 and NRRL B-18750 have dipteran activity. Thus, these cultures, or mutants thereof, can be used to control such insect pests. Further, genes encoding novel .delta.-endotoxins can be removed from these cultures and transferred to other host microbes, or plants. Expression of the .delta.-endotoxins in such hosts results in the control of susceptible insect pests in the environment of such hosts.

Bacillus thuringiensis isolates active against dipteran pests

The subject disclosure concerns Bacillus thuringiensis strains which can be used to control lepidopteran pests. The strains were previously known to control coleopteran pests. The discovery of lepidopteran activity was totally unexpected. These B.t. strains can be formulated using standard lepidopteran formulation procedures. Means of administration are also standard. The genes encoding lepidopteran-active toxins can be isolated from the B.t. isolates and used to transform other microbes for use to control lepidopteran pests.

Growing community of inventors

San Diego, CA, United States of America

Kendrick Akira Uyeda