Unitika Ltd.

Ken Iwata

Hiroshi Nakajima

Tatsuo Katayama

Kazue Kawahara

Hitoshi Kondo

Tadao Suzuki

The object of the invention is to provide a handy type visually determinable ATP measuring reagent which can be easily handled and has good measuring sensitivity. The invention is an ATP measuring reagent which rendered possible visual determination by amplifying the reaction to form glucose 6-phosphate from glucose through the joint use of ATP contained in a sample and acetyl phosphate contained in the reagent with glucokinase and acetate kinase contained in the reagent, and further leading it to a visualizable color reaction by the combination of glucose-6-phosphate dehydrogenase and diaphorase.

Method and reagent for visually measuring ATP

Test strips by which creatine kinase activity can be quantitatively measured at a high sensitivity within a broad measuring range and which have excellent storage stability. Particularly, test strips for the measurement of creatine kinase activity, which comprises a carrier, a dehydrogenase, a diaphorase, NAD or NADP, and a water-soluble tetrazolium compound.

Test strip for creatine kinase activity measurement

This invention relates to a process for the production of fructose 2,6-bisphosphate which comprises effecting reactions among (i) fructose 6-phosphate, (ii) glucose, (iii) fructose or (iv) glucose 6-phosphate, ATP and a phosphate donor, in the presence of (i) fructose 6-phosphate 2-kinase (PFK 2) and an enzyme which converts ADP into ATP (ADP/ATP converting enzyme), (ii) PFK 2, an ADP/ATP converting enzyme, hexokinase or glucokinase and glucose 6-phosphate isomerase, (iii) PFK 2, an ADP/ATP converting enzyme and hexokinase or glucokinase, or (iv) PFK 2, an ADP/ATP converting enzyme and glucose 6-phosphate isomerase; to a process for the production of fructose 2,6-bisphosphate which comprises allowing diesterase to coexist in a solution containing fructose 1,2-cyclic, 6-bisphosphate; and to a process for the purification of fructose 2,6-bisphosphate which comprises adding zinc salt to a solution containing fructose 2,6-bisphosphate, removing formed precipitate of impurities and adding a zinc salt to the resulting solution to isolate formed precipitate of fructose 2,6-bisphosphate. According to the present invention, fructose 2,6-bisphosphate can be produced easily with a high yield and high purity fructose 2,6-bisphosphate can be obtained easily.

Process for producing fructose 2,6,-bisphosphate and purification

A test strip which comprises a carrier, a dehydrogenase, diaphorase, a fluorescent chromogen, and NAD or NADP. The test strip provides high sensitivity and high accuracy measurement, and have excellent storage stability.

Growing community of inventors

Kyoto, Japan

Ken Iwata