Juridical Foundation the Chemo-sero-therapeutic Research Institute

Transgenic Inc.

Ken-ichi Yamamura

Chikateru Nozaki

Hiroshi Yonemura

Kimi Araki

Masatake Araki

Junichi Miyazaki

A trap vector containing a loxP sequence composed of inverted repeat sequence 1, a spacer sequence and inverted repeat sequence 2 in this order, the loxP sequence being a mutant loxP wherein a part of the inverted repeat sequence 1 or 2 is mutated.

Trap vectors and gene trapping method by using the same

Trap vectors and gene trapping by using the same

An expression vector for the expression of an exogenous gene in an animal cell, which contains a chick .beta.-actin gene promoter and a restriction enzyme site for incorporating an exogenous gene at the downstream of said promoter and a process for expressing an exogenous gene, which comprises incorporating said exogenous gene into the expression vector at the restriction enzyme site for incorporating the exogenous gene, introducing said vector into an animal cell and culturing the obtained transformed animal cell. The expression system of the present invention can be applied to an expression of any exogenous gene and has an extremely high expression efficiency and is applicable to a wide range of host cells, and hence can sufficiently be utilized for industrial-scale production of a useful material.

Exogenous gene expression vector containing chick .beta.-actin gene

Growing community of inventors

Kumamoto, Japan

Ken-ichi Yamamura