Beckman Coulter, Inc.

Iris International, Inc.

Ken Good

Bart Jan Wanders

Jiuliu Lu

John Riley

Bian Qian

A focusing distance (e.g., a distance between a focal plane of a camera used when n image was captured and the actual focal plane for an in-focus image) in a visual analysis system may be determined by subjecting one or more images captured by such a system to a multi-layer analysis. In such an analysis, an input may be subjected to one or more convolution filters, and the ultimate result of such convolution may be provided to a dense layer which can provide the focusing distance. This focusing distance may then be used to (re)focus a camera or for other purposes (e.g., generating an alert).

Focus quality determination through multi-layer processing

A method for dynamic focusing is presented that can be performed by a dynamic focusing controller that can receive images from the image capture device, and for each image, determine a border of the particle within the image, and calculate a pixel intensity ratio of the image based on the border of the particle. The dynamic focusing controller can also calculate a median pixel intensity ratio from the pixel intensity ratios for each image, determine a focal distance direction based on the median pixel intensity ratio, calculate a focal distance based on the median pixel intensity ratio when the focal distance direction is positive, and calculate the focal distance based on a median border width when the focal distance direction is negative. The autofocusing controller can then send an instruction to the focusing mechanism to adjust the image capture device by the focal distance in the focal distance direction.

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Glendale, CA, United States of America

Ken Good