E.i. Dupont De Nemours and Company

Kelley C Norton

James M Odom

Mattheos Koffas

Rick Weizhang Ye

Qiong Cheng

Stephen K Picataggio

Pierre E Rouviere

Jean-Francois Tomb

Andreas Schenzle

Luan Tao

J Martin Odom

Andreas J Schenzle

Genes isolated fromsp. 16a have been determined to play a role in the carotenoid biosynthetic pathway. Specifically, crtN2 gene has the ability to produce omega-aldehyde functional groups on carotenogenic substrates, while the ald gene produced omega carboxyl functional groups. These genes will be useful for production of high levels of functionalized carotenoid compounds, especially those produced in microorganisms which metabolize single carbon substrates.

Genetic constructs encoding carotenoid biosynthetic enzymes

Genes have been isolated from16a sp. encoding the isoprenoid biosynthetic pathway. The genes and gene products are the first isolated from astrain that is capable of utilizing single carbon (C1) substrates as energy sources. The genes and gene products of the present invention may be used in a variety of ways for the production of isoprenoid compounds in a variety of organisms.

Genes involved in isoprenoid compound production

This invention relates to the isolation of nucleic acid fragments from Methylamons sp. that encode enzymes involved in denitrification. The enzymes arc useful in denitrification reactions and for the identification of other denitrifying bacteria. In addition, this invention also relates to the construction of chimeric genes encoding all or a substantial portion of the present genes in sense or antisense orientation, wherein the expression of the chimeric genes results in production of altered levels of the present gene products in the recombinant host.

Genes encoding denitrification enzymes

Genes have been isolated from asp encoding enzymes in the carbon flux pathway. The genes encode a 2-keto-3-deoxy-6-phosphogluconate (KDPGA) and a fructose bisphosphate aldolase (FFBPA), as well as numerous other genes. The genes will be useful in C1 metabolizing microorganisms for the manipulation of the carbon flux pathway.

Methanotrophic carbon metabolism pathway genes and enzymes

Genes have been isolated from a Methylomonas sp encoding enzymes in the carbon flux pathway. The genes encode a 2-keto-3-deoxy-6-phosphogluconate (KDPGA) and a fructose bisphosphate aldolase (FFBPA), as well as numerous other genes. The genes will be useful in C1 metabolizing microorganisms for the manipulation of the carbon flux pathway.

Genes have been isolated from Methylomonas 16 sp. encoding the isoprenoid biosynthetic pathway. The genes and gene products are the first isolated from a Methylomonas strain that is capable of utilizing single carbon (C1) substrates as energy sources. The genes and gene products of the present invention may be used in a variety of ways for the production of isoprenoid compounds in a variety of organisms.

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Avondale, PA, United States of America

Kelley C Norton