Dow Agrosciences LLC

United States of America, As Represented by the Secretary of Agriculture

Corteva Agriscience LLC

Kelley Ann Smith

Katherine Armstrong

Donald J Merlo

Steven Robert Webb

Otto Folkerts

Aaron Todd Woosley

Dayakar Reddy Pareddy

Nicole Linda Hopkins

Timothy Denver Hey

Ning Zhou

Joseph F Petolino

William M Ainley

Krishna M Madduri

Mark Allen Thompson

Janna Mai Armstrong

Beth C Rubin-Wilson

Liu Shen

Lauren Clark

Yang Wang

Michael Ainley

Scott Belmar

Michael A Menke

Terry R Wright

Justin Michael Lira

Andrew E Robinson

Ignacio Mario Larrinua

Neil Madison Cowen

Sean Michael Russell

Nicole Arnold

Steven Lee Evans

Vipula Shukla

James Patrick Connell

Charles A Mihaliak

Kelley L Ralph

Theodore Jack Letherer

Geoffrey J Letchworth

The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified inA3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.

Selectable marker genes

Soybean Event pDAB9582.816.15.1 comprises gene expression cassettes which contain genes encoding Cry1F, Cry1Ac (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products. The disclosure provides polynucleotide related event detection methods. The present disclosure relates to a method for detecting a new insect resistant and herbicide tolerant transgenic soybean transformation event, designated Soybean Event pDAB9582.816.15,1. The DNA of soybean plants containing this event includes the junction/flanking sequences described herein that characterize the location of the inserted DNA within the soybean genome. SEQ ID NO: 1 and SEQ ID N0:2 are diagnostic for Soybean Event pDAB9582.816.15.1.

Soybean event pDAB9582.816.15.1 detection method

Soybean Event pDAB9582.814.19.1 comprises genes encoding Cry1F, Cry1Ac (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products. The invention provides PCR event detection methods.

Soybean event pDAB9582.814.19.1 detection method

This invention pertains in part to the development of a vaccine for poultry against necrotic enteritis (NE). The vaccine utilizes a protective antigen that is a mutated, full-length, non-toxic(Cp) &#x3b1;-toxin protein (Mcpa). Utility of this vaccine was demonstrated by reduction of lesion severity in NE challenge trails, for example. Also disclosed herein are novel approaches for producing this vaccine in significant quantities. One exemplified approach involves producing NE vaccine (mutated alpha toxin) in bacterial expression systems, preferably utilizing thesystem, for commercial use in controlling NE in the poultry industry. The subject vaccines can be administered preferably to chickens in several different ways. A novel, Type VI alpha toxin from chicken isolates of Cp is also disclosed.

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The subject application provides various compositions of matter directed to West Nile virus (WNV) polypeptides and fragments thereof and polynucleotides, vectors and transformed host cells that encode, direct the expression of, or produce WNV polypeptides as set forth herein. Methods of using the polypeptides and polynucleotides for the production of immune responses in individuals or detecting the presence of WNV specific or neutralizing antibodies are also provided herein.

Plant-made west nile virus (WNV) vaccines, vectors and plant codon optimized sequences

The present invention relates to nucleic acid and amino acid sequences that confer herbicide resistance in plants, as well as herbicide resistance in plants, plant seeds, plant tissues and plant cells comprising such sequences. In a preferred embodiment, the sequences of the present invention confer a tolerant phenotype in plants in response to a chronic and/or acute inhibitin dose of auxinic herbicides. The present invention also provides homologous sequences with a high degree of functional similarity.

Nucleic acid compositions conferring herbicide resistance

The present invention provides DNA sequences that function as 3' untranslated regions (3&#x2032;UTR) in plants. The 3&#x2032;UTR's stabilize associated recombinant transcripts such that expression is improved. The invention further provides plant expression cassettes and recombinant plant that comprise a claimed 3&#x2032;UTR.

Use of regulatory sequences in transgenic plants

The promoter from the maize actin depolymerizing factor gene is useful in controlling transgene expression in plants.

Promoter and intron from maize actin depolymerizing factor

The maize myo-inositol-1-phosphate synthase (MIP synthase) promoter is a novel embryo specific regulatory sequence useful in expressing heterologous nucleic acids in plants.

Maize MIP synthase promoter

Regulatory sequences derived from the maize per5 gene have utility in plant biotechnology.

Regulatory sequences for transgenic plants

This invention relates to compositions and methods useful in the production of transgenic plants. In particular, the invention relates to maize metallothionein gene transcription regulatory sequences which are useful for directing expression of heterologous DNAs in plant embryo tissue. The invention also relates to expression cassettes containing the promoter sequences and to transgenic plants containing the expression cassettes.

Regulatory sequences useful for gene expression in plant embryo tissue

Regulatory sequences derived from a maize root preferential cationic peroxidase gene (Per5), including the promoter, introns, and the 3&prime; untranslated region, are useful to control expression of recombinant genes in plants.

Growing community of inventors

Lebanon, IN, United States of America

Kelley Ann Smith