Janssen Pharmaceuticals, Inc.

Glaxosmithkline Biologicals Sa

Kellen Cristhina Fae

Jeroen Geurtsen

Eveline Marleen Weerdenburg

Michael T Kowarik

Stefan Jochen Kemmler

Manuela Mally

Darren Robert Abbanat

Patricia Ibarra Yon

Veronica Gambillara Fonck

Pieter Jan Burghout

Martin Edward Braun

Jan Theunis Poolman

Maria Paula Carranza Sandmeier

Jan Grijpstra

Louris Jakob Feitsma

Polypeptides comprising a FimH lectin domain comprising an amino acid mutation that causes the FimH lectin domain to be in the low affinity conformation for mannose are described. Pharmaceutical compositions which comprise such polypeptides and methods of stimulating an immune response in a subject in need thereof by administration of the polypeptide are further described.

FimH mutant, compositions therewith and use thereof

Methods of producing bioconjugates of O-antigen polysaccharides covalently linked to a carrier protein using recombinant host cells are provided. The recombinant host cells used in the methods described herein encode a particular oligosaccharyl transferase enzyme depending on the O-antigen polysaccharide bioconjugate to be produced. The oligosaccharyl transferase enzymes can be PglB oligosaccharyl transferase or variants thereof. Also provided are compositions containing the bioconjugates, and methods of using the bioconjugates and compositions described herein to vaccinate a subject against extra-intestinal pathogenic. (ExPEC).

Methods of producing bioconjugates ofO-antigen polysaccharides, compositions thereof, and methods of use thereof

A bioconjugate of anglucosylated O4 antigen polysaccharide covalently linked to a carrier protein and compositions thereof are provided. Also provided are recombinant host cells for producing the bioconjugate, and methods of producing the bioconjugate using the recombinant host cells. The recombinant host cells contain a nucleic acid encoding a glucosyl transferase capable of modifying theO4 antigen with glucose branching to produce the glucosylated O4 antigen polysaccharide. Bioconjugates of anglucosylated O4 antigen polysaccharide described herein can be used alone or in combination with one or more additionalO-antigen polysaccharides to induce antibodies against anglucosylated antigen, and to vaccinate a subject against extra-intestinal pathogenic(ExPEC).

Bioconjugates ofO-antigen polysaccharides, methods of production thereof, and methods of use thereof

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Mainz, Germany

Kellen Cristhina Fae