Kawasaki Jukogyo Kabushiki Kaisha

Keizo Watakabe

Katsumi Nakashima

Osamu Ohji

Takashi Sakurai

Hitoshi Hasunuma

Yohichi Nakamura

Junji Iizaka

Shimpei Ito

A cell detachment device includes: a base; a vessel holder, to which a culture vessel is to be fitted; a guide mechanism configured to guide reciprocating movement of the vessel holder; a collided member configured to collide with the vessel holder when the vessel holder is moving; an urging member configured to urge the culture vessel toward the collided member; and a power imparting mechanism configured to impart, to the vessel holder, power for causing the vessel holder to move.

Cell detachment device

Provided are a method capable of discriminating between a colony of differentiated pluripotent stem cells and a colony of undifferentiated pluripotent stem cells, and a method for automatically culturing undifferentiated pluripotent stem cells and a system thereof. A differentiated colony composed of differentiated pluripotent stem cells, an undifferentiated colony composed of undifferentiated pluripotent stem cells and a multilayered colony having pluripotent stem cells stacked in multiple layers are discriminated by processing a photo image of a colony including pluripotent stem cells in a culture vessel. Specifically, a colony having a luminance higher than a first threshold A is determined as a differentiated colony, a colony having a luminance equal to or lower than the first threshold A and equal to or higher than a second threshold B is determined as an undifferentiated colony, and a colony having a luminance lower than the second threshold B is determined as a multilayered colony.

Method and apparatus for discriminating undifferentiated pluripotent stem cells, and automated culture method and system

A method and device achieves identification on whether a colony on a cultivation container is differentiated or undifferentiated on an occasion of cultivation of multipotent stem cell, and a method and device achieves a rapid culture of undifferentiated multipotent stem cells. Undifferentiated colony containing only undifferentiated multipotent stem cells is identified from other types of colonies by means of the circularity C of the colony in an image-processed taken image of the inside of cultivation container of a multipotent stem cell. More specifically, when a first threshold of the circularity of the colony is C, and a second predetermined threshold is C(C&#x3c;C), a colony satisfying C&#x2266;C is determined as the undifferentiated colony, a colony satisfying C&#x2266;C&#x3c;Cis determined as an undefined colony, and a colony satisfying C&#x3c;Cis determined as the differentiated colony.

Method and device for identifying multipotent stem cell colony, and method and device for automatically culturing multipotent stem cells

Growing community of inventors

Kobe, Japan

Keizo Watakabe