Ishihara Sangyo Kaisha, Ltd.

Keizo Miyata

Nobutoshi Yamada

Kazuhiro Yamamoto

Hirohiko Kimura

Fuminori Kato

Masanari Kato

Yoshiyuki Aoyama

Hiroshi Shikama

Hiroyuki Ikegami

Hiromi Takeo

Medical composition containing nitroetheneamine Derivative or salt there of as active constituent

Medical composition containing nitroetheneamine derivative or salt thereof as active constituent

A polypeptide which is a tumor necrosis factor polypeptide having an amino acid sequence represented by from the 1st Ser to the 155th Leu as shown by SEQ ID NO:1 in the Sequence Listing, or its mutein, wherein the amino acid sequence of the 1st Ser to the 8th Asp of the SEQ ID NO:1 or the corresponding amino acid sequence of the mutein is replaced by an amino acid sequence containing at least one amino acid sequence of Arg-Gly-Asp and from 3 to 16 amino acids. Also disclosed are a recombinant plasmid containing a DNA encoding such a polypeptide, a recombinant microbial cell transformed by such a recombinant plasmid, a process for producing the polypeptide, a pharmaceutical composition comprising the polypeptide as an active ingredient, and a DNA for the polypeptide.

Muteins of TNF pharmaceutical compositions and a method of making

A polypeptide which is a tumor necrosis factor polypeptide having an amino acid sequence represented by from the 1st Ser to the 155th Leu as shown by SEQ ID NO:1 in the Sequence Listing, or its mutein, wherein the amino acid sequence of the 1st Ser to the 8th Asp of the SEQ ID NO:1 or the corresponding amino acid sequence of the mutein is replaced by an amino acid sequence containing at least one amino acid sequence of cell-adherent peptide of laminin and from 5 to 30 amino acids. Also disclosed are a recombinant plasmid containing a DNA encoding such a polypeptide, a recombinant microbial cell transformed by such a recombinant plasmid, a process for producing the polypeptide, a pharmaceutical composition comprising the polypeptide as an active ingredient, and a DNA for the polypeptide.

N-terminal muteins of tumor necrosis factor

Growing community of inventors

Shiga, Japan

Keizo Miyata