Promega Corporation

University of California

Promeaga Corporation

Keith V Wood

Monika G Wood

Lance P Encell

Dieter Klaubert

Mary P Hall

Thomas A Kirkland

Brock Binkowski

Thomas Machleidt

Matthew B Robers

Chad Zimprich

Georgyi V Los

Mark McDougall

Erika Hawkins

Poncho Meisenheimer

James J Cali

Paul Otto

Aldis Darzins

Michael R Slater

Melanie Dart

James Unch

Rachel Friedman Ohana

John William Shultz

Gediminas Vidugiris

Robert F Bulleit

Nidhi Nath

William Daily

Marie Schwinn

Andrew S Dixon

James Robert Hartnett

Said A Goueli

Carolyn Woodroofe Hitko

Hicham Zegzouti

Subhanjan Mondal

Dileep K Pulukkunat

Martin Ken Lewis

Martha A O'Brien

Kris Zimmerman

Samuel Kin Sang Ho

Frank Fan

Michael P Valley

Joel R Walker

Anton Shakhmin

Harry Tetsuo Uyeda

Kristopher Zimmerman

Wenhui Zhou

Donna M Leippe

Trent Gu

Michelle N Mandrekar

Roy Welch

Christine Ann Andrews

Daniel Kephart

Richard Byron Rhodes

Braeden L Butler

Richard Somberg

Susan Frackman

Kate Qin Zhao

Daniel J Simpson

Tonny Johnson

Ce Shi

Sergiy Levin

Sergei Saveliev

Jianquan Liu

Ryan J Olson

Robin Hurst

Aileen Paguio

Monika G Gruber

Michael Scurria

John M Centanni

Marjeta Urh

David W Ow

Richard A Moravec

Christopher Todd Eggers

Donald R Helinski

Jeffrey Roux De Wet

Stephen H Howell

Rita Hannah

Jacqueline Sankbeil

Marlene DeLuca McElroy, Deceased

Bruce A Sherf

Matt Robers

Ethan Edward Strauss

Susan Wigdal

Ji Zhu

Brian Almond

Yao Zhuang

Mike Scurria

Pete Stecha

Hui Wang

Michael Killoran

Trish Hoang

John W Schultz

Thomas P Smith

Tamara Sue Pratt

W D McElroy, Executor

Elaine T Schenborn

Virginia Kincaid

Samuel K Ho

Brock F Binkowski

Braeden Butler

Provided herein are isolated polynucleotide encoding modified click beetle luciferase polypeptides that have enhanced luminescence and longer wavelength near-infrared signals. The disclosure also relates to near-infrared bioluminescence systems that include said modified click beetle luciferase polypeptides and novel luciferin derivatives, as well as methods of using said modified click beetle luciferase polypeptides and bioluminescence systems.

Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence

An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-typeluciferase.

-derived luciferases, novel coelenterazine substrates, and methods of use

Described are substituted imidazo[1,2-a]pyrazine compounds, which are coelenterazine analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays. Also described are methods from making the compounds, such as a method using aminopyrazine acetophosphonates as synthesis intermediates.

Substituted imidazo[1,2-α]pyrazines as luciferase substrates

Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Activation of bioluminescence by structural complementation

Provided herein are bioluminescent polypeptides and compositions and methods for the assembly of a tripartite or multipartite bioluminescent complex. In particular embodiments, a bioluminescent complex is formed upon the interaction of three or more peptide and/or polypeptide components.

Multipartite luciferase peptides and polypeptides

An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type

Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use

The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.

Cleavable surfactants

Provided herein are compositions and methods for photoaffinity labeling of molecular targets. In particular, probes that specifically interact with cellular targets based on their affinity and are then covalently linked to the cellular target via a photoreactive group (PRG) on the probe.

Photoaffinity probes

Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).

Luciferase-based thermal shift assays

Described are processes for preparing substituted imidazo[1,2-a]pyrazine compounds of formula (I): by reacting a compound of formula (xi): with carbonyldiimidazole to form the compound of formula (I).

Process for preparing substituted imidazo[1,2-&#x3b1;]pyrazines

Provided herein are compositions and methods for detection and analysis of intracellular binding of a bioactive agent to a cellular target. In particular, bioactive agents tethered to fluorophores, cellular targets fused to bioluminescent reporters, and methods of detecting and analyzing the interaction of bioactive agents with cellular targets therewith.

Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer

Provided herein are cell-permeable, cell-compatible, and chemoselectively-cleavable linkers for tethering (e.g., covalently) functional elements (e.g., a cellular interaction element and a capture element), and methods of use (e.g., intracellular capture and extracellular release of cellular targets) therewith.

Cell-permeable, cell-compatible, and cleavable linkers for covalent tethering of functional elements

A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-typeluciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-typeluciferase of SEQ ID NO: 1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-typeluciferase.

Syntheticluciferases with enhanced light output

Growing community of inventors

Mount Horeb, WI, United States of America

Keith V Wood