Stratagene

Strategene

Keith V Sylvester

William D Huse

Anthony M Sorge

Joseph A Sorge

One aspect of the invention is to provide methods for the storage of electrophoresis gels, particularly, electrophoresis gels adapted for polynucleotide sequencing. The invention provides numerous methods of preparing a packaged electrophoresis gel or gel assembly. The subject methods include the steps of inserting an electrophoresis gel or gel assembly into the interior chamber of a storage container, removing air from the interior chamber, and hermetically sealing the storage container so as to provide a sealed package containing an electrophoresis gel in a reduced atmospheric environment. Preferably, the interior chamber is flushed with an inert gas prior to the sealing process. Another aspect of the invention is to provide packaged electrophoresis gels. The packaged electrophoresis gels include a hermetically sealed storage container having an interior chamber. The interior chamber contains a gel or gel assembly suitable for electrophoresis. The interior chamber of the storage container encloses a reduced atmospheric environment. Additionally, the interior chamber preferably contains a buffer solution. The storage container may comprise two sheets of flexible polymer which are fused to one another at or near the perimeter so as to form an interior chamber.

Methods for storage of sequencing gels and stored sequencing gels used

An apparatus for chromatography of DNA, RNA, proteins and other molecules includes the use of a column adapted to hold a chromatography material and a sample to be filtered. A pneumatic pressure differential is applied across the column and the sample is urged through the chromatography material. A selected portion of the sample may then be collected.

Push column chromatography apparatus

Push column and chromatography apparatus

Push column and chromatography method

A method for chromatography of DNA, RNA, proteins and other molecules includes the use of a column adapted to hold a chromatography material and a sample to be filtered. A pneumatic pressure differential is applied across the column and the sample is urged through the chromatography material. A selected portion of the sample may then be collected.

Push column chromatography method

A vertically oriented gel electrophoresis apparatus having a support structure for supporting in a generally vertical plane a gel plate assembly, including a pair of upright side members, a support plate lying in a vertical plane and extending between the side members, a lower buffer tank for supporting the gel plate assembly between the side members, and an upper buffer tray having a sealing gasket on one side thereof which is engageable with the gel plate assembly adjacent the upper edge thereof upon movement of the upper tray into engagement with the gel plate assembly, to seal against leakage of buffered solution disposed in the upper tray. The upper tray is moved into sealing engagement with the gel plate assembly and away therefrom by a cam disposed in cam slots in the sides of the upper tray and operated by a handle disposed externally of the side members and connected to the cam by a shaft passing through the side members. The buffer solution in the upper tray can be drained therefrom by a drain system connected through the bottom of the upper tray and connected to a portion of the lower tank. Roller retainer or locking members are slidably received by each of the side members and include a roller having resilient surface for engagement with the surface of the gel plate assembly for retaining the gel plate assembly in position against the support plate thereby minimizing mechanical stress. The gasket attached to the upper tray is configured with ribs and compartments to minimize the extent of leakage, and includes a portion positioned over the top of one of the plates of the gel plate assembly as well as portions enclosing sides thereof adjacent the upper end.

Growing community of inventors

San Diego, CA, United States of America

Keith V Sylvester