Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Other

Purdue Research Foundation

University of Arizona

Pioneer Hi-bred Int'l Inc.

Keith S Lowe

William James Gordon-Kamm

Christopher Jay Scelonge

Yumin Tao

George J Hoerster

Bo Shen

Timothy George Helentjaris

Mitchell Christian Tarczynski

Rebecca E Cahoon

Theodore Mitchell Klein

Peizhong Zheng

Carolyn A Gregory

Tim Fox

Marc C Albertsen

Yongzhong Wu

Mary Trimnell

Brian A Larkins

Matthew A Bailey

Brian R Dilkes

David J Peterson

Bailin Li

Ning Wang

Marianna Faller

Ronald D Burnett

Kevin E McBride

Dennis L Bidney

Ramgopal Nadimpalli

Sonriza Rasco-Gaunt

Young Min Woo

Bing-Bing Wang

Xifan Sun

Carl R Simmons

James Jefferson Smith

Derek Jantz

Shane E Abbitt

Joshua K Young

Huirong Gao

Leszek Aleksander Lyznik

Stanton B Gelvin

Sergei Svitashev

John A McElver

Margit C Ross

Grace Marie St Clair

Carlos M La Rota

Yuejin Sun

Huaxun Ye

Jon Aaron Tucker Reinders

Mike Lassner

Stephen Lawrence Gasior

Saikat Bhattacharjee

Saverio Carl Falco

Anthony John Kinney

Andrew Mark Cigan

Dwight T Tomes

Omolayo O Famodu

Benjamin A Bowen

Wesley B Bruce

Kevin L Stecca

Scott E Nichols

Jinrui Shi

Leslie T Harvell

Carl W Garnaat

William B Allen

Gary A Sandahl

Changjiang Li

Shiv Bahadur Tiwari

Bradley Allen Roth

Craig E Hastings

Xinli Emily Wu

Susan J Martino-Catt

Sheila E Maddock

Kathryn K Lappegard

Igor Cunha Oliveira

Ajith Anand

Lisa Newman

David D Songstad

Laura A Church

Carl Falco

Mark Cigan

Todd J Jones

Aurélie Morin

Grace M St Clair

Kimberly F Glassman

Patrea M Hill

Ricardo A Dante

Rachel Carol Huegel

Lawrence R Stiner

Maren L Arling

Tim W Fox

Kevin E Mcbride

Carolyn Ann Gregory

Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.

Use of morphogenic factors for the improvement of gene editing

The methods disclosed herein provide for clonal plant production using a morphogenic gene to produce non-transgenic and transgenic plants wherein the morphogenic gene does not integrate into the genome of the plant.

Methods for clonal plant production

Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.

Methods and compositions for the introduction and regulated expression of genes in plants

Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.

Systems and methods for cellular reprogramming of a plant cell

The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

Methods and compositions for rapid plant transformation

Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences for regulatory regions. Phospholipid transfer protein (PLTP) promoters from maize are provided. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence, such as a maize PLTP promoter, disclosed herein. DNA constructs comprising a promoter operably linked to a heterologous nucleotide sequence of interest are also provided.

Embryo-preferredpromoters and methods of use

Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.

AP2 domain transcription factor ODP2 (Ovule Development Protein 2) and methods of use

AP2 domain transcription factor ODP2 (ovule development protein 2) and methods of use

A variety of methods and compositions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism.

Methods and compositions for targeted polynucleotide modification

A variety of methods and compostions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excize a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism.

Growing community of inventors

Johnston, IA, United States of America

Keith S Lowe