Commonwealth Scientific and Industrial Research Organisation

Other

Biotechnology Australia Pty, Ltd

Keith Norman Rand

Peter Laurence Molloy

Gary Stewart Cobon

Alagacone Sriskantha

David Harold Kemp

Peter Willadsen

George Alfred Riding

Joanna Terry Moore

Law A Johnston

Law Anthony Yorke Johnston

A method for detecting a nucleic acid molecule having a target sequence adjacent a 3' terminus is provided. Also provided is a method for differentiating nucleic acid molecules having a target sequence adjacent a 3&#x2032; terminus from nucleic acid molecules in which the same sequence is embedded within the molecule.

Amplification of DNA fragments

Provided herein is a method for the selective amplification of a target nucleotide sequence located within a nucleic acid molecule, the method comprising contacting the nucleic acid molecule ('template' molecule) with (i) at least one facilitator oligonucleotide, wherein the facilitator oligonucleotide includes at least one modification at or near its 3' terminus such that 3&#x2032; extension from the facilitator oligonucleotide is blocked, and (ii) two or more oligonucleotide primers, at least one of which is an initiator primer modified such that the presence of the modification prematurely terminates complementary strand synthesis, wherein the facilitator oligonucleotide and the initiator primer bind to substantially the same or adjacent regions of the template nucleic acid molecule and the facilitator oligonucleotide further comprises sequences complementary to the target sequence 3&#x2032; to the binding location of the initiator primer; and carrying out thermocyclic, enzymatic amplification such that the specific target sequence is selectively amplified.

Nucleic acid amplification

A method for the selective amplification of a target nucleic acid in a sample comprising the target nucleic acid and at least one non-target nucleic acid, the method comprising amplifying the nucleic acids by means of at least one oligonucleotide primer comprising:

Headloop DNA amplification

This invention relates to an antigen isolated from the cattle tick,and to the gene coding for that antigen and to the protein product of that gene. The antigen when used in part or in entirety as an immunogen administered to cattle as a vaccine results in the production by the cattle of an immune response which is capable of damaging ticks feeding on vaccinated cattle to such an extent that the survival of such ticks is decreased and/or the reproductive capacity of the ticks is decreased to such an extent that the antigen coded for by the gene can be used as an effective vaccine against said ticks.

DNA encoding a cell membrane glycoprotein of a tick gut

The invention relates to antigens derived from ticks and to their purification. It also relates to genes encoding such antigens and to their cloning and expression from recombinant DNA molecules. Further, the invention describes the use of purified antigens and recombinant expression products having similar biological activity to those purified antigens to provide vaccines to protect cattle against tick infestation.

Growing community of inventors

Frenchs Forest, Australia

Keith Norman Rand