454 Life Sciences Corporation

Keith McDade

John W Simpson

Jonathan Marc Rothberg

Jan Berka

Joel S Bader

Scott B Dewell

Christopher M Colangelo

Yi-Ju Chen

John Harris Leamon

Michael Weiner

Joseph M Fierro

James R Knight

Jaran Charumilind

Eugene W Myers, Jr

Greg A Volkmer

Jane Berka

Michael Philip Weiner

An embodiment of method for correcting an error associated with phasic synchrony of sequence data generated from a population of substantially identical copies of a template molecule is described that comprises (a) detecting a signal generated in response to an incorporation of one or more nucleotides in a sequencing reaction; (b) generating a value for the signal; and (c) correcting the value for the phasic synchrony error using a first parameter and a second carry forward parameter.

System and method for correcting primer extension errors in nucleic acid sequence data

An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.

Methods of amplifying and sequencing nucleic acids

Disclosed herein are methods and apparatuses for sequencing a nucleic acid. These methods permit a very large number of independent sequencing reactions to be arrayed in parallel, permitting simultaneous sequencing of a very large number (&#x3e;10,000) of different oligonucleotides.

Apparatus and method for sequencing a nucleic acid

Method of sequencing a nucleic acid

Disclosed herein are methods and apparatuses for sequencing a nucleic acid. In one aspect, the method includes annealing a population of circular nucleic acid molecules to a plurality of anchor primers linked to a solid support, and amplifying those members of the population of circular nucleic acid molecules which anneal to the target nucleic acid, and then sequencing the amplified molecules by detecting the presence of a sequence byproduct such as pyrophosphate.

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Clinton, CT, United States of America

Keith McDade