Pfenex Inc.

Dow Global Technolgoies LLC

Keith L Haney

Lawrence C Chew

Jeffrey Allen

Anant Patkar

Ping-Hua Feng

Lei Lei Phokham Sengchanthalangsy

Richard S Harner

Timm R Richardson

Torben R Bruck

Lei Lei Sengchanthalangsy

The present invention relates to the field of recombinant protein production in bacterial hosts. It further relates to extraction of soluble, active recombinant protein from an insoluble fraction without the use of denaturation and without the need for a refolding step. In particular, the present invention relates to a production process for obtaining high levels a soluble recombinant Type 1 interferon protein from a bacterial host.

Method for producing soluble recombinant interferon protein without denaturing

A method for monitoring fluid media, such as a dynamic biological system, in a biological reactor containing developing culture fluid media. The method includes the step of directing light into the fluid media by way of one or more optical fibers to produce an illuminated fluid media and then measuring the intensity of light reflected from the illuminated fluid media by way of one or more optical fibers, the optical fibers being partitioned from the fluid media by a transparent window having inner and outer surfaces; the fibers having ends terminating adjacent to and confronting the inner surface of the window and extending in a direction away from the window, the corresponding ends of the fibers being radially and circumferentially spaced from one another, the corresponding ends of the fibers having converging and intersecting longitudinal projections therefrom, the intersecting longitudinal projections from the fibers being entirely within the window. Additionally, a fiber-optic probe is discussed.

Fiber-optic probes and methods of measuring biological materials

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San Diego, CA, United States of America

Keith L Haney