Bruker Cellular Analysis, Inc.

Keith Joseph Breinlinger

Mark P White

Eric D Hobbs

J Tanner Nevill

Daniele Malleo

Andrew W McFarland

John A Tenney

Aathavan Karunakaran

Randall D Lowe, Jr

Kevin T Chapman

Jason M McEwen

Gang F Wang

Justin K Valley

Kristin G Beaumont

Christopher C Shing

Dorian Liepmann

Angel Navas Angeles

Raziel Solomon Alon

Russell A Newstrom

Joshua Tanner Nevill

Xiao Guan

Natalie C Marks

Jonathan Cloud Dragon Hubbard

Patrick N Ingram

Erin Chia-wei Hsi

John Junyeon Kim

Maria Jimena Loureiro

Yogesh Khemchandra Dhande

Andrew W Mcfarland

Paul M Lundquist

Systems for operating a microfluidic device are described. The systems comprise a first surface configured to interface and operatively couple with a microfluidic device and a lid configured to retain the microfluidic device on the first surface. The lid comprises a first portion having a first fluid port configured to operatively couple with and flow fluidic medium into and/or out of a first fluid inlet/outlet of the microfluidic device and a second portion having a second fluid port configured to operatively couple with and flow fluidic medium into and/or out of a second fluid inlet/outlet of the microfluidic device. The second portion of the lid is separable from the first portion and movable between a closed position in which the second fluid port of the second portion of the cover is operatively coupled with the second fluid inlet/outlet of the microfluidic device and an open position in which a portion of the microfluidic device that contains the second fluid inlet/outlet is exposed. Other embodiments are described.

Systems for operating microfluidic devices

Microfluidic devices having a circuit substrate with a control unit, a switching mechanism associated with a dielectrophoresis (DEP) electrode, and a memory unit are described. Switching instructions may be received, stored, and retrieved by the control unit and used to control the DEP electrode via the switching mechanism. Systems comprising the described microfluidic devices and methods of controlling the described microfluidic devices are included herein.

Microfluidic device with programmable switching elements

Some configurations of a microfluidic apparatus can comprise a fluidic circuit of interconnected fluidic structures into which a plurality of different media can be introduced or extracted. A variety of operations can be performed with the different media including isolating with a second medium one or more of the fluidic structures that is filled partially or fully with a first medium. Discrete volumes of a medium can be moved through the isolating second medium to deliver materials or micro-objects to or remove micro-objects or materials from a fluidic structure that is otherwise isolated by the second medium. Some configurations of a microfluidic apparatus can isolate microfluidic structures in a microfluidic apparatus using flow rates or blocking structures, and some configurations can manage bubbles in fluidic structures.

Isolating microfluidic structures and trapping bubbles

Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

Microfluidic cell culture

Growing community of inventors

San Rafael, CA, United States of America

Keith Joseph Breinlinger