Berkeley Lights, Inc.

Formfactor, Inc.

Teradyne, Inc.

Other

Phenomex Inc.

Keith J Breinlinger

J Tanner Nevill

Eric D Hobbs

Andrew W McFarland

Daniele Malleo

Mark P White

Benjamin N Eldridge

Alexander H Slocum, Jr

Michael J Stone

Gaetan L Mathieu

Arash Behziz

Pavan Ramkumar

Aathavan Karunakaran

Randall D Lowe, Jr

John K Gritters

Kevin T Chapman

Frank Parrish

Jason M McEwen

James R Varney

Troy A Lionberger

Volker L S Kurz

Gang F Wang

Xiaohua Wang

Kristin G Beaumont

James M Ormond

Christopher C Shing

Matthew C White

Bryan R Hotaling

Paul M Lebel

Brandon R Bruhn

Natalie C Marks

Xiao Guan

Kevin J Hughes

Edward J Milovic

Kai W Szeto

Paul M Lundquist

Ronald A Sartschev

John A Tenney

Michael J Armstrong

Justin K Valley

Douglas S Ondricek

Tommie Edward Berry

Gerald H Johnson

Brian Brecht

Nicholas J Teneketges

Edward A Ostertag

Eric Sackmann

Russell A Newstrom

Shawn Powell

Marc Loranger

Derek Castellano

Arthur E LeColst

Thomas M Vetterli

Donald Eric Thompson

David Pritzkau

Bruce L Cowgill

Darshan Thaker

Kevin P Manning

Vincent Haw Tien Pai

Hayley M Bennett

Kevin Youl Yasumura

Eric K Sackmann

Adrian S Wilson

David Evans

Christoph Andreas Neyer

Elisabeth Marie Walczak

Alexander Gerald Olson

Wesley Arthur Zink

Jonathan Cloud Dragon Hubbard

Oleksandr Tokmakov

Igor Fastnacht

Yuriy Nicheporuk

Andriy Koval

Russell Newstrom

Khrystyna Andres

Alona Kostenko

Andrew Mcfarland

Alexander Slocum

Gaetan Mathieu

Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.

Microfluidic devices for optically-driven convection and displacement, kits and methods thereof

Display screen with graphical user interface

Disclosed are methods, systems, and articles of manufacture for performing a process on biological samples. An analysis of biological samples in multiple regions of interest in a microfluidic device and a timeline correlated with the analysis may be identified. One or more region-of-interest types for the multiple regions of interest may be determined; and multiple characteristics may be determined for the biological samples based at least in part upon the one or more region-of-interest types. Associated data that respectively correspond to the multiple regions of interest in a user interface for at least a portion of the biological samples in the user interface based at least in part upon the multiple identifiers and the timeline. A count of the biological samples in a region of interest may be determined based at least in part upon a class or type of data using a convolutional neural network (CNN).

Systems and methods for analyses of biological samples

Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

Microfluidic cell culture

A microfluidic device can comprise a plurality of interconnected microfluidic elements. A plurality of actuators can be positioned abutting, immediately adjacent to, and/or attached to deformable surfaces of the microfluidic elements. The actuators can be selectively actuated and de-actuated to create directed flows of a fluidic medium in the microfluidic (or nanofluidic) device. Further, the actuators can be selectively actuated and de-actuated to create localized flows of a fluidic medium in the microfluidic device to move reagents and/or micro-objects in the microfluidic device.

Actuated microfluidic structures for directed flow in a microfluidic device and methods of use thereof

A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.

Movement and selection of micro-objects in a microfluidic apparatus

A group of micro-objects in a holding pen in a micro-fluidic device can be selected and moved to a staging area, from which the micro-objects can be exported from the micro-fluidic device. The micro-fluidic device can have a plurality of holding pens, and each holding pen can isolate micro-objects located in the holding pen from micro-objects located in the other holding pens or elsewhere in the micro-fluidic device. The selected group of micro-objects can comprise one or more biological cells, such as a clonal population of cells. Embodiments of the invention can thus select a particular group of clonal cells in a micro-fluidic device, move the clonal cells to a staging area, and export the clonal cells from the micro-fluidic device while maintaining the clonal nature of the exported group.

Exporting a selected group of micro-objects from a micro-fluidic device

Analytical instrument

A system for operating an electrokinetic device includes a support configured to hold and operatively couple with the electrokinetic device, an integrated electrical signal generation subsystem configured to apply a biasing voltage across a pair of electrodes in the electrokinetic device, and a light modulating subsystem configured to emit structured light onto the electrokinetic device. The system can further include a thermally controlled flow controller, and/or be configured to measure impedance across the electrokinetic device. The system can be a light microscope, including an optical train. The system can further include a light pipe, which can be part of the light modulating subsystem, and which can be configured to supply light of substantially uniform intensity to the light modulating subsystem or directly to the optical train.

Systems for operating electrokinetic devices

Elongated flexible probes can be disposed in holes of upper and lower guide plates of a probe card assembly. Each probe can include one or more spring mechanisms that exert normal forces against sidewalls of holes in one of the guide plates. The normal forces can result in frictional forces against the sidewalls that are substantially parallel to the sidewalls. The frictional forces can reduce or impede movement parallel to the sidewalls of the probes in the holes.

Probes with spring mechanisms for impeding unwanted movement in guide holes

Growing community of inventors

San Ramon, CA, United States of America

Keith J Breinlinger